LOCUS       BBa_K1747000             955 bp    DNA     linear       17-May-2024
DEFINITION  Diagnostic circuit using GFP to evaluate fatty acid promoter 
            repsonse
ACCESSION   BBa_K1747000
VERSION     BBa_K1747000.1  
FEATURES             Location/Qualifiers
     promoter        1..72
                     /label=PfadBA
     promoter        1..72
                     /label=BBa_K817002
     misc_feature    85..88
                     /label=conserved
     RBS             81..92
                     /label=BBa_B0034
     CDS             99..818
                     /label=GFP protein
     CDS             99..818
                     /label=BBa_E0040
     stem_loop       838..881
                     /label=stem_loop
     misc_feature    827..906
                     /label=BBa_B0010
     terminator      827..906
                     /label=BBa_B0010
     stem_loop       922..941
                     /label=T7 TE
     misc_feature    948..948
                     /label=stop
     polyA_site      942..955
                     /label=polya
     misc_feature    915..955
                     /label=BBa_B0012
     terminator      915..955
                     /label=BBa_B0012
     terminator      827..955
                     /label=BBa_B0015
ORIGIN
        1 atctggtacg accagatttg acaatctggt acgaccagat gatactgagc acatcagcag
       61 gacgcactga cctactagag aaagaggaga aatactagat gcgtaaagga gaagaacttt
      121 tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt
      181 ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt aaatttattt
      241 gcactactgg aaaactacct gttccatggc caacacttgt cactactttc ggttatggtg
      301 ttcaatgctt tgcgagatac ccagatcata tgaaacagca tgactttttc aagagtgcca
      361 tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg aactacaaga
      421 cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag ttaaaaggta
      481 ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac tataactcac
      541 acaatgtata catcatggca gacaaacaaa agaatggaat caaagttaac ttcaaaatta
      601 gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa aatactccaa
      661 ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa tctgcccttt
      721 cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta acagctgctg
      781 ggattacaca tggcatggat gaactataca aataataata ctagagccag gcatcaaata
      841 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
      901 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//