LOCUS       BBa_K1747008            2599 bp    DNA     linear       09-May-2024
DEFINITION  Fatty acid promoter - GFP - crtZ gene - term - lacl - fadR gene - 
            term
ACCESSION   BBa_K1747008
VERSION     BBa_K1747008.1  
FEATURES             Location/Qualifiers
     promoter        1..72
                     /label=PfadBA
     promoter        1..72
                     /label=BBa_K817002
     misc_feature    77..80
                     /label=conserved
     RBS             73..84
                     /label=BBa_B0034
     misc_feature    1..84
                     /label=BBa_K1747004
     CDS             91..810
                     /label=GFP protein
     CDS             91..810
                     /label=BBa_E0040
     RBS             819..834
                     /label=BBa_I742156
     CDS             841..1368
                     /label=crtZ
     CDS             841..1371
                     /label=BBa_I742157
     stem_loop       1391..1434
                     /label=stem_loop
     misc_feature    1380..1459
                     /label=BBa_B0010
     terminator      1380..1459
                     /label=BBa_B0010
     stem_loop       1475..1494
                     /label=T7 TE
     misc_feature    1501..1501
                     /label=stop
     polyA_site      1495..1508
                     /label=polya
     misc_feature    1468..1508
                     /label=BBa_B0012
     terminator      1468..1508
                     /label=BBa_B0012
     terminator      1380..1508
                     /label=BBa_B0015
     CDS             1517..1604
                     /label=end of LacI coding region (inactive)
     misc_feature    1517..1716
                     /label=BBa_R0010
     misc_feature    1605..1642
                     /label=CAP binding site
     promoter        1653..1658
                     /label=-35
     promoter        1677..1682
                     /label=-10
     misc_feature    1682..1716
                     /label=LacI binding site
     misc_feature    1689..1689
                     /label=start
     promoter        1517..1716
                     /label=BBa_R0010
     misc_feature    1729..1732
                     /label=conserved
     RBS             1725..1736
                     /label=BBa_B0034
     misc_feature    1517..1736
                     /label=BBa_J04500
     CDS             1743..2462
                     /label=FadR
     CDS             1743..2462
                     /label=BBa_K817001
     misc_feature    1517..2462
                     /label=BBa_K817007
     stem_loop       2482..2525
                     /label=stem_loop
     misc_feature    2471..2550
                     /label=BBa_B0010
     terminator      2471..2550
                     /label=BBa_B0010
     stem_loop       2566..2585
                     /label=T7 TE
     misc_feature    2592..2592
                     /label=stop
     polyA_site      2586..2599
                     /label=polya
     misc_feature    2559..2599
                     /label=BBa_B0012
     terminator      2559..2599
                     /label=BBa_B0012
     terminator      2471..2599
                     /label=BBa_B0015
ORIGIN
        1 atctggtacg accagatttg acaatctggt acgaccagat gatactgagc acatcagcag
       61 gacgcactga ccaaagagga gaaatactag atgcgtaaag gagaagaact tttcactgga
      121 gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt
      181 ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact
      241 ggaaaactac ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc
      301 tttgcgagat acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa
      361 ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct
      421 gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt
      481 aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc acacaatgta
      541 tacatcatgg cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac
      601 attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat
      661 ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat
      721 cccaacgaaa agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca
      781 catggcatgg atgaactata caaataataa tactagagct ctaccggaga aatttactag
      841 atgttgtgga tttggaatgc cctgatcgtt ttcgttaccg tgattggcat ggaagtgatt
      901 gctgcactgg cacacaaata catcatgcac ggctggggtt ggggatggca tctttcacat
      961 catgaaccgc gtaaaggtgc gtttgaagtt aacgatcttt atgccgtggt ttttgctgca
     1021 ttatcgatcc tgctgattta tctgggcagt acaggaatgt ggccgctcca gtggattggc
     1081 gcaggtatga cggcgtatgg attactctat tttatggtgc acgacgggct ggtgcatcaa
     1141 cgttggccat tccgctatat tccacgcaag ggctacctca aacggttgta tatggcgcac
     1201 cgtatgcatc acgccgtcag gggcaaagaa ggttgtgttt cttttggctt cctctatgcg
     1261 ccgcccctgt caaaacttca ggcgacgctc cgggaaagac atggcgctag agcgggcgct
     1321 gccagagatg cgcagggcgg ggaggatgag cccgcatccg ggaagtaata atactagagc
     1381 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     1441 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     1501 cgtttatata ctagagcaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     1561 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     1621 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     1681 ttgtgtggaa ttgtgagcgg ataacaattt cacacatact agagaaagag gagaaatact
     1741 agatggtcat taaggcgcaa agcccggcgg gtttcgcgga agagtacatt attgaaagta
     1801 tctggaataa ccgcttccct cccgggacta ttttgcccgc agaacgtgaa ctttcagaat
     1861 taattggcgt aacgcgtact acgttacgtg aagtgttaca gcgtctggca cgagatggct
     1921 ggttgaccat tcaacatggc aagccgacga aggtgaataa tttctgggaa acttccggtt
     1981 taaatatcct tgaaacactg gcgcgactgg atcacgaaag tgtgccgcag cttattgata
     2041 atttgctgtc ggtgcgtacc aatatttcca ctatttttat tcgcaccgcg tttcgtcagc
     2101 atcccgataa agcgcaggaa gtgctggcta ccgctaatga agtggccgat cacgccgatg
     2161 cctttgccga gctggattac aacatattcc gcggcctggc gtttgcttcc ggcaacccga
     2221 tttacggtct gattcttaac gggatgaaag ggctgtatac gcgtattggt cgtcactatt
     2281 tcgccaatcc ggaagcgcgc agtctggcgc tgggcttcta ccacaaactg tcggcgttgt
     2341 gcagtgaagg cgcgcacgat caggtgtacg aaacagtgcg tcgctatggg catgagagtg
     2401 gcgagatttg gcaccggatg cagaaaaatc tgccgggtga tttagccatt caggggcgat
     2461 aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     2521 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
     2581 gggcctttct gcgtttata
//