LOCUS       BBa_K1747009            2038 bp    DNA     linear       11-May-2024
DEFINITION  fatty acid promoter - GFP - term - lacl - fadR gene - term
ACCESSION   BBa_K1747009
VERSION     BBa_K1747009.1  
FEATURES             Location/Qualifiers
     promoter        1..72
                     /label=PfadBA
     promoter        1..72
                     /label=BBa_K817002
     misc_feature    77..80
                     /label=conserved
     RBS             73..84
                     /label=BBa_B0034
     misc_feature    1..84
                     /label=BBa_K1747004
     CDS             91..810
                     /label=GFP protein
     CDS             91..810
                     /label=BBa_E0040
     stem_loop       830..873
                     /label=stem_loop
     misc_feature    819..898
                     /label=BBa_B0010
     terminator      819..898
                     /label=BBa_B0010
     stem_loop       914..933
                     /label=T7 TE
     misc_feature    940..940
                     /label=stop
     polyA_site      934..947
                     /label=polya
     misc_feature    907..947
                     /label=BBa_B0012
     terminator      907..947
                     /label=BBa_B0012
     terminator      819..947
                     /label=BBa_B0015
     CDS             956..1043
                     /label=end of LacI coding region (inactive)
     misc_feature    956..1155
                     /label=BBa_R0010
     misc_feature    1044..1081
                     /label=CAP binding site
     promoter        1092..1097
                     /label=-35
     promoter        1116..1121
                     /label=-10
     misc_feature    1121..1155
                     /label=LacI binding site
     misc_feature    1128..1128
                     /label=start
     promoter        956..1155
                     /label=BBa_R0010
     misc_feature    1168..1171
                     /label=conserved
     RBS             1164..1175
                     /label=BBa_B0034
     misc_feature    956..1175
                     /label=BBa_J04500
     CDS             1182..1901
                     /label=FadR
     CDS             1182..1901
                     /label=BBa_K817001
     misc_feature    956..1901
                     /label=BBa_K817007
     stem_loop       1921..1964
                     /label=stem_loop
     misc_feature    1910..1989
                     /label=BBa_B0010
     terminator      1910..1989
                     /label=BBa_B0010
     stem_loop       2005..2024
                     /label=T7 TE
     misc_feature    2031..2031
                     /label=stop
     polyA_site      2025..2038
                     /label=polya
     misc_feature    1998..2038
                     /label=BBa_B0012
     terminator      1998..2038
                     /label=BBa_B0012
     terminator      1910..2038
                     /label=BBa_B0015
ORIGIN
        1 atctggtacg accagatttg acaatctggt acgaccagat gatactgagc acatcagcag
       61 gacgcactga ccaaagagga gaaatactag atgcgtaaag gagaagaact tttcactgga
      121 gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt
      181 ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact
      241 ggaaaactac ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc
      301 tttgcgagat acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa
      361 ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct
      421 gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt
      481 aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc acacaatgta
      541 tacatcatgg cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac
      601 attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat
      661 ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat
      721 cccaacgaaa agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca
      781 catggcatgg atgaactata caaataataa tactagagcc aggcatcaaa taaaacgaaa
      841 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
      901 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac tagagcaata
      961 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     1021 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     1081 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     1141 taacaatttc acacatacta gagaaagagg agaaatacta gatggtcatt aaggcgcaaa
     1201 gcccggcggg tttcgcggaa gagtacatta ttgaaagtat ctggaataac cgcttccctc
     1261 ccgggactat tttgcccgca gaacgtgaac tttcagaatt aattggcgta acgcgtacta
     1321 cgttacgtga agtgttacag cgtctggcac gagatggctg gttgaccatt caacatggca
     1381 agccgacgaa ggtgaataat ttctgggaaa cttccggttt aaatatcctt gaaacactgg
     1441 cgcgactgga tcacgaaagt gtgccgcagc ttattgataa tttgctgtcg gtgcgtacca
     1501 atatttccac tatttttatt cgcaccgcgt ttcgtcagca tcccgataaa gcgcaggaag
     1561 tgctggctac cgctaatgaa gtggccgatc acgccgatgc ctttgccgag ctggattaca
     1621 acatattccg cggcctggcg tttgcttccg gcaacccgat ttacggtctg attcttaacg
     1681 ggatgaaagg gctgtatacg cgtattggtc gtcactattt cgccaatccg gaagcgcgca
     1741 gtctggcgct gggcttctac cacaaactgt cggcgttgtg cagtgaaggc gcgcacgatc
     1801 aggtgtacga aacagtgcgt cgctatgggc atgagagtgg cgagatttgg caccggatgc
     1861 agaaaaatct gccgggtgat ttagccattc aggggcgata atactagagc caggcatcaa
     1921 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     1981 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//