LOCUS BBa_K1747012 7156 bp DNA linear 10-May-2024 DEFINITION Fatty acid promoter - GFP - crt Z,E,B,I,Y - term - Repressor circuit ACCESSION BBa_K1747012 VERSION BBa_K1747012.1 FEATURES Location/Qualifiers promoter 1..72 /label=PfadBA promoter 1..72 /label=BBa_K817002 misc_feature 77..80 /label=conserved RBS 73..84 /label=BBa_B0034 misc_feature 1..84 /label=BBa_K1747004 CDS 91..810 /label=GFP protein CDS 91..810 /label=BBa_E0040 RBS 819..834 /label=BBa_I742156 CDS 835..1362 /label=crtZ CDS 835..1365 /label=BBa_I742157 misc_feature 819..1365 /label=BBa_I742158 RBS 1374..1377 /label=BBa_K118012 CDS 1384..2295 /label=BBa_I742151 misc_feature 2304..2306 /label=SacI RBS 2307..2313 /label=rbs RBS 2304..2313 /label=BBa_J15001 CDS 2320..3249 /label=crtB coding sequence CDS 2320..3252 /label=BBa_K118002 misc_feature 3261..3263 /label=SacI RBS 3264..3270 /label=rbs RBS 3261..3270 /label=BBa_J15001 misc_feature 3754..3759 /label=PstI site removed misc_feature 4165..4170 /label=PstI site removed CDS 3277..4755 /label=crtI coding sequence CDS 3277..4758 /label=BBa_K118003 RBS 4767..4770 /label=BBa_K118012 CDS 4777..5925 /label=crtY coding sequence CDS 4777..5928 /label=BBa_K118008 stem_loop 5948..5991 /label=stem_loop misc_feature 5937..6016 /label=BBa_B0010 terminator 5937..6016 /label=BBa_B0010 stem_loop 6032..6051 /label=T7 TE misc_feature 6058..6058 /label=stop polyA_site 6052..6065 /label=polya misc_feature 6025..6065 /label=BBa_B0012 terminator 6025..6065 /label=BBa_B0012 terminator 5937..6065 /label=BBa_B0015 misc_feature 1..6065 /label=BBa_K1747011 CDS 6074..6161 /label=end of LacI coding region (inactive) misc_feature 6074..6273 /label=BBa_R0010 misc_feature 6162..6199 /label=CAP binding site promoter 6210..6215 /label=-35 promoter 6234..6239 /label=-10 misc_feature 6239..6273 /label=LacI binding site misc_feature 6246..6246 /label=start promoter 6074..6273 /label=BBa_R0010 misc_feature 6286..6289 /label=conserved RBS 6282..6293 /label=BBa_B0034 misc_feature 6074..6293 /label=BBa_J04500 CDS 6300..7019 /label=FadR CDS 6300..7019 /label=BBa_K817001 misc_feature 6074..7019 /label=BBa_K817007 stem_loop 7039..7082 /label=stem_loop misc_feature 7028..7107 /label=BBa_B0010 terminator 7028..7107 /label=BBa_B0010 stem_loop 7123..7142 /label=T7 TE misc_feature 7149..7149 /label=stop polyA_site 7143..7156 /label=polya misc_feature 7116..7156 /label=BBa_B0012 terminator 7116..7156 /label=BBa_B0012 terminator 7028..7156 /label=BBa_B0015 ORIGIN 1 atctggtacg accagatttg acaatctggt acgaccagat gatactgagc acatcagcag 61 gacgcactga ccaaagagga gaaatactag atgcgtaaag gagaagaact tttcactgga 121 gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt 181 ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact 241 ggaaaactac ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc 301 tttgcgagat acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa 361 ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct 421 gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt 481 aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc acacaatgta 541 tacatcatgg cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac 601 attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat 661 ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat 721 cccaacgaaa agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca 781 catggcatgg atgaactata caaataataa tactagagct ctaccggaga aattatgttg 841 tggatttgga atgccctgat cgttttcgtt accgtgattg gcatggaagt gattgctgca 901 ctggcacaca aatacatcat gcacggctgg ggttggggat ggcatctttc acatcatgaa 961 ccgcgtaaag gtgcgtttga agttaacgat ctttatgccg tggtttttgc tgcattatcg 1021 atcctgctga tttatctggg cagtacagga atgtggccgc tccagtggat tggcgcaggt 1081 atgacggcgt atggattact ctattttatg gtgcacgacg ggctggtgca tcaacgttgg 1141 ccattccgct atattccacg caagggctac ctcaaacggt tgtatatggc gcaccgtatg 1201 catcacgccg tcaggggcaa agaaggttgt gtttcttttg gcttcctcta tgcgccgccc 1261 ctgtcaaaac ttcaggcgac gctccgggaa agacatggcg ctagagcggg cgctgccaga 1321 gatgcgcagg gcggggagga tgagcccgca tccgggaagt aataatacta gaggaggtac 1381 tagatgacgg tctgcgcaaa aaaacacgtt catctcactc gcgatgctgc ggagcagtta 1441 ctggctgata ttgatcgacg ccttgatcag ttattgcccg tggagggaga acgggatgtt 1501 gtgggtgccg cgatgcgtga aggtgcgctg gcaccgggaa aacgtattcg ccccatgttg 1561 ctgttgctga ccgcccgcga tctgggttgc gctgtcagcc atgacggatt actggatttg 1621 gcctgtgcgg tggaaatggt ccacgcggct tcgctgatcc ttgacgatat gccctgcatg 1681 gacgatgcga agctgcggcg cggacgccct accattcatt ctcattacgg agagcatgtg 1741 gcaatactgg cggcggttgc cttgctgagt aaagcctttg gcgtaattgc cgatgcagat 1801 ggcctcacgc cgctggcaaa aaatcgggcg gtttctgaac tgtcaaacgc catcggcatg 1861 caaggattgg ttcagggtca gttcaaggat ctgtctgaag gggataagcc gcgcagcgct 1921 gaagctattt tgatgacgaa tcactttaaa accagcacgc tgttttgtgc ctccatgcag 1981 atggcctcga ttgttgcgaa tgcctccagc gaagcgcgtg attgcctgca tcgtttttca 2041 cttgatcttg gtcaggcatt tcaactgctg gacgatttga ccgatggcat gaccgacacc 2101 ggtaaggata gcaatcagga cgccggtaaa tcgacgctgg tcaatctgtt aggcccgagg 2161 gcggttgaag aacgtctgag acaacatctt cagcttgcca gtgagcatct ctctgcggcc 2221 tgccaacacg ggcacgccac tcaacatttt attcaggcct ggtttgacaa aaaactcgct 2281 gccgtcagtt aataatacta gagctcaagg aggtactaga tgaataatcc gtcgttactc 2341 aatcatgcgg tcgaaacgat ggcagttggc tcgaaaagtt ttgcgacagc ctcaaagtta 2401 tttgatgcaa aaacccggcg cagcgtactg atgctctacg cctggtgccg ccattgtgac 2461 gatgttattg acgatcagac gctgggcttt caggcccggc agcctgcctt acaaacgccc 2521 gaacaacgtc tgatgcaact tgagatgaaa acgcgccagg cctatgcagg atcgcagatg 2581 cacgaaccgg cgtttgcggc ttttcaggaa gtggctatgg ctcatgatat cgccccggct 2641 tacgcgtttg atcatctgga aggcttcgcc atggatgtac gcgaagcgca atacagccaa 2701 ctggatgata cgctgcgcta ttgctatcac gttgcaggcg ttgtcggctt gatgatggcg 2761 caaatcatgg gcgtgcggga taacgccacg ctggaccgcg cctgtgacct tgggctggca 2821 tttcagttga ccaatattgc tcgcgatatt gtggacgatg cgcatgcggg ccgctgttat 2881 ctgccggcaa gctggctgga gcatgaaggt ctgaacaaag agaattatgc ggcacctgaa 2941 aaccgtcagg cgctgagccg tatcgcccgt cgtttggtgc aggaagcaga accttactat 3001 ttgtctgcca cagccggcct ggcagggttg cccctgcgtt ccgcctgggc aatcgctacg 3061 gcgaagcagg tttaccggaa aataggtgtc aaagttgaac aggccggtca gcaagcctgg 3121 gatcagcggc agtcaacgac cacgcccgaa aaattaacgc tgctgctggc cgcctctggt 3181 caggccctta cttcccggat gcgggctcat cctccccgcc ctgcgcatct ctggcagcgc 3241 ccgctctaat aatactagag ctcaaggagg tactagatga aaccaactac ggtaattggt 3301 gcaggcttcg gtggcctggc actggcaatt cgtctacaag ctgcggggat ccccgtctta 3361 ctgcttgaac aacgtgataa acccggcggt cgggcttatg tctacgagga tcaggggttt 3421 acctttgatg caggcccgac ggttatcacc gatcccagtg ccattgaaga actgtttgca 3481 ctggcaggaa aacagttaaa agagtatgtc gaactgctgc cggttacgcc gttttaccgc 3541 ctgtgttggg agtcagggaa ggtctttaat tacgataacg atcaaacccg gctcgaagcg 3601 cagattcagc agtttaatcc ccgcgatgtc gaaggttatc gtcagtttct ggactattca 3661 cgcgcggtgt ttaaagaagg ctatctaaag ctcggtactg tccctttttt atcgttcaga 3721 gacatgcttc gcgccgcacc tcaactggcg aaactgcaag catggagaag cgtttacagt 3781 aaggttgcca gttacatcga agatgaacat ctgcgccagg cgttttcttt ccactcgctg 3841 ttggtgggcg gcaatccctt cgccacctca tccatttata cgttgataca cgcgctggag 3901 cgtgagtggg gcgtctggtt tccgcgtggc ggcaccggcg cattagttca ggggatgata 3961 aagctgtttc aggatctggg tggcgaagtc gtgttaaacg ccagagtcag ccatatggaa 4021 acgacaggaa acaagattga agccgtgcat ttagaggacg gtcgcaggtt cctgacgcaa 4081 gccgtcgcgt caaatgcaga tgtggttcat acctatcgcg acctgttaag ccagcaccct 4141 gccgcggtta agcagtccaa caaactgcaa actaagcgca tgagtaactc tctgtttgtg 4201 ctctattttg gtttgaatca ccatcatgat cagctcgcgc atcacacggt ttgtttcggc 4261 ccgcgttacc gcgagctgat tgacgaaatt tttaatcatg atggcctcgc agaggacttc 4321 tcactttatc tgcacgcgcc ctgtgtcacg gattcgtcac tggcgcctga aggttgcggc 4381 agttactatg tgttggcgcc ggtgccgcat ttaggcaccg cgaacctcga ctggacggtt 4441 gaggggccaa aactacgcga ccgtattttt gcgtaccttg agcagcatta catgcctggc 4501 ttacggagtc agctggtcac gcaccggatg tttacgccgt ttgattttcg cgaccagctt 4561 aatgcctatc atggctcagc cttttctgtg gagcccgttc ttacccagag cgcctggttt 4621 cggccgcata accgcgataa aaccattact aatctctacc tggtcggcgc aggcacgcat 4681 cccggcgcag gcattcctgg cgtcatcggc tcggcaaaag cgacagcagg tttgatgctg 4741 gaggatctga tataataata ctagaggagg tactagatgc aaccgcatta tgatctgatt 4801 ctcgtggggg ctggactcgc gaatggcctt atcgccctgc gtcttcagca gcagcaacct 4861 gatatgcgta ttttgcttat cgacgccgca ccccaggcgg gcgggaatca tacgtggtca 4921 tttcaccacg atgatttgac tgagagccaa catcgttgga tagctccgct ggtggttcat 4981 cactggcccg actatcaggt acgctttccc acacgccgtc gtaagctgaa cagcggctac 5041 ttttgtatta cttctcagcg tttcgctgag gttttacagc gacagtttgg cccgcacttg 5101 tggatggata ccgcggtcgc agaggttaat gcggaatctg ttcggttgaa aaagggtcag 5161 gttatcggtg cccgcgcggt gattgacggg cggggttatg cggcaaattc agcactgagc 5221 gtgggcttcc aggcgtttat tggccaggaa tggcgattga gccacccgca tggtttatcg 5281 tctcccatta tcatggatgc cacggtcgat cagcaaaatg gttatcgctt cgtgtacagc 5341 ctgccgctct cgccgaccag attgttaatt gaagacacgc actatattga taatgcgaca 5401 ttagatcctg aatgcgcgcg gcaaaatatt tgcgactatg ccgcgcaaca gggttggcag 5461 cttcagacac tgctgcgaga agaacagggc gccttaccca ttactctgtc gggcaatgcc 5521 gacgcattct ggcagcagcg ccccctggcc tgtagtggat tacgtgccgg tctgttccat 5581 cctaccaccg gctattcact gccgctggcg gttgccgtgg ccgaccgcct gagtgcactt 5641 gatgtcttta cgtcggcctc aattcaccat gccattacgc attttgcccg cgagcgctgg 5701 cagcagcagg gctttttccg catgctgaat cgcatgctgt ttttagccgg acccgccgat 5761 tcacgctggc gggttatgca gcgtttttat ggtttacctg aagatttaat tgcccgtttt 5821 tatgcgggaa aactcacgct gaccgatcgg ctacgtattc tgagcggcaa gccgcctgtt 5881 ccggtattag cagcattgca agccattatg acgactcatc gttaataata ctagagccag 5941 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 6001 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt 6061 ttatatacta gagcaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc 6121 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg 6181 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg 6241 tgtggaattg tgagcggata acaatttcac acatactaga gaaagaggag aaatactaga 6301 tggtcattaa ggcgcaaagc ccggcgggtt tcgcggaaga gtacattatt gaaagtatct 6361 ggaataaccg cttccctccc gggactattt tgcccgcaga acgtgaactt tcagaattaa 6421 ttggcgtaac gcgtactacg ttacgtgaag tgttacagcg tctggcacga gatggctggt 6481 tgaccattca acatggcaag ccgacgaagg tgaataattt ctgggaaact tccggtttaa 6541 atatccttga aacactggcg cgactggatc acgaaagtgt gccgcagctt attgataatt 6601 tgctgtcggt gcgtaccaat atttccacta tttttattcg caccgcgttt cgtcagcatc 6661 ccgataaagc gcaggaagtg ctggctaccg ctaatgaagt ggccgatcac gccgatgcct 6721 ttgccgagct ggattacaac atattccgcg gcctggcgtt tgcttccggc aacccgattt 6781 acggtctgat tcttaacggg atgaaagggc tgtatacgcg tattggtcgt cactatttcg 6841 ccaatccgga agcgcgcagt ctggcgctgg gcttctacca caaactgtcg gcgttgtgca 6901 gtgaaggcgc gcacgatcag gtgtacgaaa cagtgcgtcg ctatgggcat gagagtggcg 6961 agatttggca ccggatgcag aaaaatctgc cgggtgattt agccattcag gggcgataat 7021 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt 7081 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg 7141 cctttctgcg tttata //