LOCUS       BBa_K1747016            2238 bp    DNA     linear       11-May-2024
DEFINITION  pLR + hpd + gfp
ACCESSION   BBa_K1747016
VERSION     BBa_K1747016.1  
FEATURES             Location/Qualifiers
     promoter        1..72
                     /label=PfadBA
     promoter        1..72
                     /label=BBa_K817002
     misc_feature    85..88
                     /label=conserved
     RBS             81..92
                     /label=BBa_B0034
     CDS             99..1355
                     /label=BBa_K1747013
     misc_feature    1368..1371
                     /label=conserved
     RBS             1364..1375
                     /label=BBa_B0034
     CDS             1382..2101
                     /label=GFP protein
     CDS             1382..2101
                     /label=BBa_E0040
     stem_loop       2121..2164
                     /label=stem_loop
     misc_feature    2110..2189
                     /label=BBa_B0010
     terminator      2110..2189
                     /label=BBa_B0010
     stem_loop       2205..2224
                     /label=T7 TE
     misc_feature    2231..2231
                     /label=stop
     polyA_site      2225..2238
                     /label=polya
     misc_feature    2198..2238
                     /label=BBa_B0012
     terminator      2198..2238
                     /label=BBa_B0012
     misc_feature    1364..2238
                     /label=BBa_I13504
ORIGIN
        1 atctggtacg accagatttg acaatctggt acgaccagat gatactgagc acatcagcag
       61 gacgcactga cctactagag aaagaggaga aatactagat gtgtctatcg ttagcttcta
      121 cagctcaacg aaacacacag ttccgtagca gagttttagt tttagcagag ttggtgaaat
      181 caatgggcca ccaaaacgcc gccgtttcag agaatcaaaa ccatgatgac ggcgctgcgt
      241 cgtcgccggg attcaagctc gtcggatttt ccaagttcgt aagaaagaat ccaaagtctg
      301 ataaattcaa ggttaagcgc ttccatcaca tcgagttctg gtgcggcgac gcaaccaacg
      361 tcgctcgtcg cttctcctgg ggtctgggga tgagattctc cgccaaatcc gatctttcca
      421 ccggaaacat ggttcacgcc tcttacctac tcacctccgg tgacctccga ttccttttca
      481 ctgctcctta ctctccgtct ctctccgccg gagagattaa accgacaacc acagcttcta
      541 tcccaagttt cgatcacggc tcttgtcgtt ccttcttctc ttcacatggt ctcggtgtta
      601 gagccgttgc gattgaagta gaagacgcag agtcagcttt ctccatcagt gtagctaatg
      661 gcgctattcc ttcgtcgcct cctatcgtcc tcaatgaagc agttacgatc gctgaggtta
      721 aactatacgg cgatgttgtt ctccgatatg ttagttacaa agcagaagat accgaaaaat
      781 ccgagttctt gccagggttc gagcgtgtag aggatgcgtc gtcgttccca ttggattatg
      841 gtatccggcg gcttgaccac gccgtgggaa acgttcctga gcttggtccg gctttaactt
      901 atgtagcggg gttcactggt tttcaccaat tcgcagagtt cacagcagac gacgttggaa
      961 ccgccgagag cggtttaaat tcagcggtcc tggctagcaa tgatgaaatg gttcttctac
     1021 cgattaacga gccagtgcac ggaacaaaga ggaagagtca gattcagacg tatttggaac
     1081 ataacgaagg cgcagggcta caacatctgg ctctgatgag tgaagacata ttcaggaccc
     1141 tgagagagat gaggaagagg agcagtattg gaggattcga cttcatgcct tctcctccgc
     1201 ctacttacta ccagaatctc aagaaacggg tcggcgacgt gctcagcgat gatcagatca
     1261 aggagtgtga ggaattaggg attcttgtag acagagatga tcaagggacg ttgcttcaaa
     1321 tcttcacaaa accactaggc cgacgatatt tatagtacta gagaaagagg agaaatacta
     1381 gatgcgtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg
     1441 tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg
     1501 aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact
     1561 tgtcactact ttcggttatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca
     1621 gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt
     1681 caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt
     1741 taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa
     1801 attggaatac aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg
     1861 aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga
     1921 ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta
     1981 cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct
     2041 tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat acaaataata
     2101 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     2161 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
     2221 ggcctttctg cgtttata
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