LOCUS       BBa_K175022              967 bp    DNA     linear       01-Jun-2024
DEFINITION  PTet+Lock3c+GFP+TT
ACCESSION   BBa_K175022
VERSION     BBa_K175022.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    63..104
                     /label=BBa_J23031
     CDS             111..830
                     /label=GFP protein
     CDS             111..830
                     /label=BBa_E0040
     stem_loop       850..893
                     /label=stem_loop
     misc_feature    839..918
                     /label=BBa_B0010
     terminator      839..918
                     /label=BBa_B0010
     stem_loop       934..953
                     /label=T7 TE
     misc_feature    960..960
                     /label=stop
     polyA_site      954..967
                     /label=polya
     misc_feature    927..967
                     /label=BBa_B0012
     terminator      927..967
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaactagaa tcacctcttg cttttgggta agacgaagag gagatactag atgcgtaaag
      121 gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg
      181 ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc
      241 ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactt
      301 tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag catgactttt
      361 tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg
      421 ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg
      481 agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca
      541 actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagtta
      601 acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac
      661 aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac
      721 aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg
      781 taacagctgc tgggattaca catggcatgg atgaactata caaataataa tactagagcc
      841 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
      901 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
      961 gtttata
//