LOCUS       BBa_K175024             1415 bp    DNA     linear       12-May-2024
DEFINITION  PLacI+Key3c+TT+PLambdain+RBS+GFP+TT
ACCESSION   BBa_K175024
VERSION     BBa_K175024.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    209..302
                     /label=BBa_J23008
     stem_loop       322..365
                     /label=stem_loop
     misc_feature    311..390
                     /label=BBa_B0010
     terminator      311..390
                     /label=BBa_B0010
     stem_loop       406..425
                     /label=T7 TE
     misc_feature    432..432
                     /label=stop
     polyA_site      426..439
                     /label=polya
     misc_feature    399..439
                     /label=BBa_B0012
     terminator      399..439
                     /label=BBa_B0012
     misc_feature    503..516
                     /label=OR1 434
     misc_feature    456..472
                     /label=OR1 lambda
     misc_feature    480..496
                     /label=OR2 lambda
     promoter        518..523
                     /label=-10
     promoter        495..500
                     /label=-35
     promoter        448..529
                     /label=BBa_I12006
     RBS             538..552
                     /label=RBS-1\Strong
     RBS             545..549
                     /label=RBS
     misc_feature    538..552
                     /label=BBa_B0030
     RBS             538..552
                     /label=BBa_B0030
     CDS             559..1278
                     /label=GFP protein
     CDS             559..1278
                     /label=BBa_E0040
     stem_loop       1298..1341
                     /label=stem_loop
     misc_feature    1287..1366
                     /label=BBa_B0010
     terminator      1287..1366
                     /label=BBa_B0010
     stem_loop       1382..1401
                     /label=T7 TE
     misc_feature    1408..1408
                     /label=stop
     polyA_site      1402..1415
                     /label=polya
     misc_feature    1375..1415
                     /label=BBa_B0012
     terminator      1375..1415
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagac ccaaaagcaa gaggtgattc tagttggtgg
      241 ttaatgaaaa ttaacttact tactagaaat atctctaaaa agccagatta ttaatccggc
      301 tttactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
      361 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
      421 gggcctttct gcgtttatat actagaggca accattatca ccgccagagg taaaatagtc
      481 aacacgcacg gtgttagata ttacaaactt tcttgtatag atttaacgtt actagagatt
      541 aaagaggaga aatactagat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt
      601 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa
      661 ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct
      721 gttccatggc caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac
      781 ccagatcata tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag
      841 gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt
      901 gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga
      961 aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca
     1021 gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga
     1081 agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt
     1141 ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag
     1201 agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat
     1261 gaactataca aataataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
     1321 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
     1381 tggctcacct tcgggtgggc ctttctgcgt ttata
//