LOCUS BBa_K175043 3066 bp DNA linear 29-May-2024 DEFINITION Inducible Self Destructive Plasmid + inducible GFP-LVA reporter ACCESSION BBa_K175043 VERSION BBa_K175043.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 RBS 209..223 /label=RBS-1\Strong RBS 216..220 /label=RBS misc_feature 209..223 /label=BBa_B0030 RBS 209..223 /label=BBa_B0030 CDS 230..977 /label=SceI CDS 230..977 /label=BBa_K142200 stem_loop 997..1040 /label=stem_loop misc_feature 986..1065 /label=BBa_B0010 terminator 986..1065 /label=BBa_B0010 stem_loop 1081..1100 /label=T7 TE misc_feature 1107..1107 /label=stop polyA_site 1101..1114 /label=polya misc_feature 1074..1114 /label=BBa_B0012 terminator 1074..1114 /label=BBa_B0012 misc_feature 1123..1141 /label=TetR 1 misc_feature 1123..1176 /label=BBa_R0040 promoter 1142..1147 /label=-35 misc_feature 1148..1166 /label=TetR 2 promoter 1165..1170 /label=-10 promoter 1123..1176 /label=BBa_R0040 misc_feature 1185..1214 /label=I-SceI homing endonuclease restriction site misc_feature 1185..1214 /label=BBa_K175027 misc_feature 1..1214 /label=BBa_K175040 RBS 1223..1235 /label=RBS-3\Weak RBS 1229..1232 /label=RBS misc_feature 1223..1235 /label=BBa_B0032 RBS 1223..1235 /label=BBa_B0032 misc_feature 1242..1244 /label=start misc_feature 1995..1997 /label=stop misc_feature 1998..2000 /label=stop misc_feature 1958..1994 /label=LVA tag CDS 1245..1957 /label=cds misc_feature 1242..2000 /label=BBa_K145015 stem_loop 2020..2063 /label=stem_loop misc_feature 2009..2088 /label=BBa_B0010 terminator 2009..2088 /label=BBa_B0010 stem_loop 2104..2123 /label=T7 TE misc_feature 2130..2130 /label=stop polyA_site 2124..2137 /label=polya misc_feature 2097..2137 /label=BBa_B0012 terminator 2097..2137 /label=BBa_B0012 misc_feature 1223..2137 /label=BBa_K175038 misc_feature 2146..2175 /label=I-SceI homing endonuclease restriction site misc_feature 2146..2175 /label=BBa_K175027 promoter 2184..2218 /label=BBa_J23116 misc_feature 2231..2234 /label=conserved RBS 2227..2238 /label=BBa_B0034 CDS 2248..2864 /label=tetR misc_feature 2865..2898 /label=SsrA misc_feature 2905..2929 /label=Help:Barcodes CDS 2245..2929 /label=BBa_C0040 stem_loop 2949..2992 /label=stem_loop misc_feature 2938..3017 /label=BBa_B0010 terminator 2938..3017 /label=BBa_B0010 stem_loop 3033..3052 /label=T7 TE misc_feature 3059..3059 /label=stop polyA_site 3053..3066 /label=polya misc_feature 3026..3066 /label=BBa_B0012 terminator 3026..3066 /label=BBa_B0012 terminator 2938..3066 /label=BBa_B0015 misc_feature 2184..3066 /label=BBa_K145201 misc_feature 2146..3066 /label=BBa_K175039 misc_feature 1223..3066 /label=BBa_K175042 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagat taaagaggag aaatactaga tgcatcaaaa 241 aaaccaggta atgaacctgg gtccgaactc taaactgctg aaagaataca aatcccagct 301 gatcgaactg aacatcgaac agttcgaagc aggtatcggt ctgatcctgg gtgatgctta 361 catccgttct cgtgatgaag gtaaaaccta ctgtatgcag ttcgagtgga aaaacaaagc 421 atacatggac cacgtatgtc tgctgtacga tcagtgggta ctgtccccgc cgcacaaaaa 481 agaacgtgtt aaccacctgg gtaacctggt aatcacctgg ggcgcccaga ctttcaaaca 541 ccaagccttc aacaaactgg ctaacctgtt catcgttaac aacaaaaaaa ccatcccgaa 601 caacctggtt gaaaactacc tgaccccgat gtctctggca tactggttca tggatgatgg 661 tggtaaatgg gattacaaca aaaactctac caacaaatcg atcgtactga acacccagtc 721 tttcactttc gaagaagtag aatacctggt taagggtctg cgtaacaaat tccaactgaa 781 ctgttacgta aaaatcaaca aaaacaaacc gatcatctac atcgattcta tgtcttacct 841 gatcttctac aacctgatca aaccgtacct gatcccgcag atgatgtaca aactgccgaa 901 cactatctcc tccgaaactt tccttaagag gcccgctgca aacgacgaaa actacgcttt 961 agtagcttaa gtaataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa 1021 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact 1081 ggctcacctt cgggtgggcc tttctgcgtt tatatactag agtccctatc agtgatagag 1141 attgacatcc ctatcagtga tagagatact gagcactact agagagttac gctagggata 1201 acagggtaat atagtactag agtcacacag gaaagtacta gatgcgtaaa ggagaagaac 1261 ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat 1321 tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta 1381 tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact ttcggttatg 1441 gtgttcaatg ctttgcgaga tacccagatc atatgaaaca gcatgacttt ttcaagagtg 1501 ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca 1561 agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag 1621 gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac aactataact 1681 cacacaatgt atacatcatg gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa 1741 ttagacacaa cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc 1801 caattggcga tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc 1861 tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg 1921 ctgggattac acatggcatg gatgaactat acaaaaggcc tgctgcaaac gacgaaaact 1981 acgctttagt agcttaataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg 2041 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac 2101 actggctcac cttcgggtgg gcctttctgc gtttatatac tagagagtta cgctagggat 2161 aacagggtaa tatagtacta gagttgacag ctagctcagt cctagggact atgctagcta 2221 ctagagaaag aggagaaata ctagatgtcc agattagata aaagtaaagt gattaacagc 2281 gcattagagc tgcttaatga ggtcggaatc gaaggtttaa caacccgtaa actcgcccag 2341 aagctaggtg tagagcagcc tacattgtat tggcatgtaa aaaataagcg ggctttgctc 2401 gacgccttag ccattgagat gttagatagg caccatactc acttttgccc tttagaaggg 2461 gaaagctggc aagatttttt acgtaataac gctaaaagtt ttagatgtgc tttactaagt 2521 catcgcgatg gagcaaaagt acatttaggt acacggccta cagaaaaaca gtatgaaact 2581 ctcgaaaatc aattagcctt tttatgccaa caaggttttt cactagagaa tgcattatat 2641 gcactcagcg ctgtggggca ttttacttta ggttgcgtat tggaagatca agagcatcaa 2701 gtcgctaaag aagaaaggga aacacctact actgatagta tgccgccatt attacgacaa 2761 gctatcgaat tatttgatca ccaaggtgca gagccagcct tcttattcgg ccttgaattg 2821 atcatatgcg gattagaaaa acaacttaaa tgtgaaagtg ggtccgctgc aaacgacgaa 2881 aactacgctt tagtagctta ataacactga tagtgctagt gtagatcact actagagcca 2941 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt 3001 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg 3061 tttata //