LOCUS BBa_K175048 1084 bp DNA linear 02-Jun-2024 DEFINITION GFP generator with medium RBS (B0032) ACCESSION BBa_K175048 VERSION BBa_K175048.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 RBS 209..221 /label=RBS-3\Weak RBS 215..218 /label=RBS misc_feature 209..221 /label=BBa_B0032 RBS 209..221 /label=BBa_B0032 CDS 228..947 /label=GFP protein CDS 228..947 /label=BBa_E0040 stem_loop 967..1010 /label=stem_loop misc_feature 956..1035 /label=BBa_B0010 terminator 956..1035 /label=BBa_B0010 stem_loop 1051..1070 /label=T7 TE misc_feature 1077..1077 /label=stop polyA_site 1071..1084 /label=polya misc_feature 1044..1084 /label=BBa_B0012 terminator 1044..1084 /label=BBa_B0012 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagtc acacaggaaa gtactagatg cgtaaaggag 241 aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc 301 acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta 361 aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc actactttcg 421 gttatggtgt tcaatgcttt gcgagatacc cagatcatat gaaacagcat gactttttca 481 agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa gatgacggga 541 actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat agaatcgagt 601 taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg gaatacaact 661 ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc aaagttaact 721 tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat tatcaacaaa 781 atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat 841 ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt gagtttgtaa 901 cagctgctgg gattacacat ggcatggatg aactatacaa ataataatac tagagccagg 961 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg 1021 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt 1081 tata //