LOCUS       BBa_K1751002            2328 bp    DNA     linear       17-May-2024
DEFINITION  Promotor-RBS+RgPAL1+Terminator
ACCESSION   BBa_K1751002
VERSION     BBa_K1751002.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     RBS             44..58
                     /label=RBS-1\Strong
     RBS             51..55
                     /label=RBS
     misc_feature    44..58
                     /label=BBa_B0030
     RBS             44..58
                     /label=BBa_B0030
     misc_feature    1..58
                     /label=BBa_K847100
     CDS             65..2191
                     /label=PAL coding region
     CDS             65..2191
                     /label=BBa_K1751000
     stem_loop       2211..2254
                     /label=stem_loop
     misc_feature    2200..2279
                     /label=BBa_B0010
     terminator      2200..2279
                     /label=BBa_B0010
     stem_loop       2295..2314
                     /label=T7 TE
     misc_feature    2321..2321
                     /label=stop
     polyA_site      2315..2328
                     /label=polya
     misc_feature    2288..2328
                     /label=BBa_B0012
     terminator      2288..2328
                     /label=BBa_B0012
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagattaaag aggagaaata
       61 ctagatggag aatgggcacc accactcgaa cgggttgtgc gtggagacta cgcgtgatcc
      121 gttgaactgg gtggcggcgg cggagtcgct gaaggggagc cacctggacg aggtgaagag
      181 gatggtggag gagttcagga agccggcggt gaagctcggc ggtgagagcc tgactatagc
      241 gcaggtggcg gcgatcgcgg cgagggataa tgcggtggcg gtggagctgg cggagacggc
      301 gcgtgcgggg gttaaggcga gtagcgattg ggttatggag agtatgaata aagggactga
      361 cagttatgga gttacaacgg gttttggtgc cacgtcacat aggaggacta aacaaggtgg
      421 tgctcttcag aaggagctca ttaggttctt gaatgccgga atattcggca acggcacgga
      481 atctaaccac gcgctgccac actccgccac gagagccgcc atgctcgtcc gaatcaacac
      541 gctcctccaa ggatattccg gcatccgatt tgaaatccta gaagccctaa caaaattcct
      601 caaccacaac atcaccccct gtttgcccct ccgcggcacg atcaccgcct ccggcgacct
      661 cgtcccgcta tcctacattg ccgggctttt aacgggccgg cccaactcca aggccgtcgg
      721 cccaaacggc gaagccctca acgccggcga ggctttcagc ctcgccggcg ttagcggctt
      781 cttcgagctg caacccaaag aaggcctcgc gctagtcaac gggacagctg tcgggtccgg
      841 attggcctcg atcgccctgt acgacgcgaa catcctcgcc gtcctgtcgg aagtgacgtc
      901 agtgattttc gctgaggtca tgaatgggaa acctgaattt acggatcatt tgacacataa
      961 gctgaaacat caccctggcc aaattgaggc cgctgctata atggaacaca ttttagatgg
     1021 tagcgcgtac gttaaggctg ctcagaaatt gcacgaaacc gatccgttgc aaaaaccgaa
     1081 acaggatcgg tacgcgctta gaacgtcgcc tcaatggctc ggcccccaaa tcgaagttat
     1141 ccgaaccgcg acgaaaatga tcgagcggga aattaattcg gttaacgaca cacctctaat
     1201 cgatgtctcg agaaataaag cgttacatgg cggtaacttc cagggcacgc caatcggggt
     1261 atcgatggac aacaccagat tggcgatagc agctatcgga aaattgatgt tcgctcaatt
     1321 ttccgagctg gttaatgatt tctacaacaa tggattgccg tctaatctct ctggcggtag
     1381 gaatccgagc ttggattacg gtttcaaagg gtccgaaatc gcgatggctt cgtattgttc
     1441 ggagcttcaa tttttagcta atcctgttac caatcatgtc caaagtgcag agcaacataa
     1501 ccaagatgtg aactcacttg gattgatttc ttccagaaag accgtcgagg ctctggatat
     1561 tctaaagctg atgtcatcca catatttaat cgcgctatgc caggccgtcg atttgaggca
     1621 cttggaggag aatttgaggc tttcagttaa aaacaccgtt agccaagtgg cgaagaggac
     1681 tctgacaatg ggtattaatg gcgaacttca tccgtcaaga ttctgcgaga aggatcttct
     1741 ccgtgtggtg gaccgcgagt acgtgtttgc atacatcgac gatccgtgca gcgggaccta
     1801 ccccttgatg cagaagttga ggcaagttct cgtggaccac gcgttgaaca acggtgagag
     1861 tgagaaaaac gtgagcacgt ctatttttca aaagatcgag gcgtttgagg tagagttgaa
     1921 ggcgatcttg cctaaagagg tcgagagtgc acggatcgcg ttggagagtg gaaatccggc
     1981 gattggtaac aggattacgg aatgcagatc gtatccgttg tacaagttta tcagagagga
     2041 acttgggacg aactacttga cgggcgaaaa ggtcgtttct ccgggggagg aatgtgataa
     2101 ggtgttcaca gctttgagca agggtttgat tgttgatcct ttgttgaagt gtcttgaggg
     2161 ttggaatggt gcacctcccc ctatctgcta gtactagagc caggcatcaa ataaaacgaa
     2221 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     2281 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//