BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1702
1
RBS
range1702
1
8
12
BBa_K1751000
1
BBa_K1751000
RgPAL1：phenylalanine ammonia lyase (PAL) coding region
2015-08-02T11:00:00Z
2015-08-19T04:47:14Z
Rehmannia glutinosa.
The gene RgPAL1 can express Phenylalanine ammonia lyase（PAL）, which is an??enzyme??that??catalyzes??a reaction converting L-phenylalanine??to ammonia??and??trans-cinnamic acid.PAL is the first and committed step in the??phenyl propanoid pathway??and is therefore involved in the??biosynthesis??of the??polyphenol??compounds. In our project, PAL plays an important role in L-phenylalanine???s deaminization to produce trans-cinnamic acid.
false
false
_2173_
28395
28395
9
false
The gene RgPAL1 can express Phenylalanine ammonia lyase（PAL）.
false
Tianlai Xia
annotation2434421
1
PAL coding region
range2434421
1
1
2127
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_K1751002
1
BBa_K1751002
Promotor-RBS+RgPAL1+Terminator
2015-08-15T11:00:00Z
2015-12-16T01:48:14Z
iGEM's database and Rehmannia glutinosa Genome
Promotor-RBS:This part contains a constitutive promoter and strong RBS. The strength of this RBS is considered relative to BBa_B0031, BBa_B0032, BBa_B0033 and BBa_B0034. While the promotor is a member of a family of constitutive promotor parts labelled J23100 through J23119.
RgPAL1:The gene RgPAL1 can express Phenylalanine ammonia lyase（PAL）, which is an enzyme that catalyzes a reaction converting L-phenylalanine to ammonia and trans-cinnamic acid.PAL is the first and committed step in the phenyl propanoid pathway and is therefore involved in the biosynthesis of the polyphenol compounds. In our project, PAL plays an important role in L-phenylalanine???s deaminization to produce trans-cinnamic acid.
false
false
_2173_
4206
28395
9
false
Promotor-RBS+RgPAL1
false
Tianlai Xia
component2436450
1
BBa_B0012
component2436447
1
BBa_K1751000
component2436445
1
BBa_K847100
component2436448
1
BBa_B0010
annotation2436445
1
BBa_K847100
range2436445
1
1
58
annotation2436447
1
BBa_K1751000
range2436447
1
65
2191
annotation2436450
1
BBa_B0012
range2436450
1
2288
2328
annotation2436448
1
BBa_B0010
range2436448
1
2200
2279
BBa_K847100
1
BBa_K847100
Promotor-RBS
2012-09-28T11:00:00Z
2015-05-08T01:13:34Z
http://partsregistry.org/Part:BBa_B0030
http://partsregistry.org/Part:BBa_J23100
Released HQ 2013
This part contains a constitutive promoter and strong RBS. The strength of this RBS is considered relative to BBa_B0031, BBa_B0032, BBa_B0033 and BBa_B0034. While the promotor is a member of a family of constitutive promotor parts labelled J23100 through J23119.
false
false
_1107_
0
12286
9
In stock
false
None
false
Bella A Okiddy, Julia Borden, Michelle Yu
component2210121
1
BBa_B0030
component2210119
1
BBa_J23100
annotation2210119
1
BBa_J23100
range2210119
1
1
35
annotation2210121
1
BBa_B0030
range2210121
1
44
58
BBa_J23100
1
BBa_J23100
constitutive promoter family member
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
Isolated from library of promoters
Released HQ 2013
Replace later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
BBa_J23100_sequence
1
ttgacggctagctcagtcctaggtacagtgctagc
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K1751000_sequence
1
atggagaatgggcaccaccactcgaacgggttgtgcgtggagactacgcgtgatccgttgaactgggtggcggcggcggagtcgctgaaggggagccacctggacgaggtgaagaggatggtggaggagttcaggaagccggcggtgaagctcggcggtgagagcctgactatagcgcaggtggcggcgatcgcggcgagggataatgcggtggcggtggagctggcggagacggcgcgtgcgggggttaaggcgagtagcgattgggttatggagagtatgaataaagggactgacagttatggagttacaacgggttttggtgccacgtcacataggaggactaaacaaggtggtgctcttcagaaggagctcattaggttcttgaatgccggaatattcggcaacggcacggaatctaaccacgcgctgccacactccgccacgagagccgccatgctcgtccgaatcaacacgctcctccaaggatattccggcatccgatttgaaatcctagaagccctaacaaaattcctcaaccacaacatcaccccctgtttgcccctccgcggcacgatcaccgcctccggcgacctcgtcccgctatcctacattgccgggcttttaacgggccggcccaactccaaggccgtcggcccaaacggcgaagccctcaacgccggcgaggctttcagcctcgccggcgttagcggcttcttcgagctgcaacccaaagaaggcctcgcgctagtcaacgggacagctgtcgggtccggattggcctcgatcgccctgtacgacgcgaacatcctcgccgtcctgtcggaagtgacgtcagtgattttcgctgaggtcatgaatgggaaacctgaatttacggatcatttgacacataagctgaaacatcaccctggccaaattgaggccgctgctataatggaacacattttagatggtagcgcgtacgttaaggctgctcagaaattgcacgaaaccgatccgttgcaaaaaccgaaacaggatcggtacgcgcttagaacgtcgcctcaatggctcggcccccaaatcgaagttatccgaaccgcgacgaaaatgatcgagcgggaaattaattcggttaacgacacacctctaatcgatgtctcgagaaataaagcgttacatggcggtaacttccagggcacgccaatcggggtatcgatggacaacaccagattggcgatagcagctatcggaaaattgatgttcgctcaattttccgagctggttaatgatttctacaacaatggattgccgtctaatctctctggcggtaggaatccgagcttggattacggtttcaaagggtccgaaatcgcgatggcttcgtattgttcggagcttcaatttttagctaatcctgttaccaatcatgtccaaagtgcagagcaacataaccaagatgtgaactcacttggattgatttcttccagaaagaccgtcgaggctctggatattctaaagctgatgtcatccacatatttaatcgcgctatgccaggccgtcgatttgaggcacttggaggagaatttgaggctttcagttaaaaacaccgttagccaagtggcgaagaggactctgacaatgggtattaatggcgaacttcatccgtcaagattctgcgagaaggatcttctccgtgtggtggaccgcgagtacgtgtttgcatacatcgacgatccgtgcagcgggacctaccccttgatgcagaagttgaggcaagttctcgtggaccacgcgttgaacaacggtgagagtgagaaaaacgtgagcacgtctatttttcaaaagatcgaggcgtttgaggtagagttgaaggcgatcttgcctaaagaggtcgagagtgcacggatcgcgttggagagtggaaatccggcgattggtaacaggattacggaatgcagatcgtatccgttgtacaagtttatcagagaggaacttgggacgaactacttgacgggcgaaaaggtcgtttctccgggggaggaatgtgataaggtgttcacagctttgagcaagggtttgattgttgatcctttgttgaagtgtcttgagggttggaatggtgcacctccccctatctgctag
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_K1751002_sequence
1
ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagatggagaatgggcaccaccactcgaacgggttgtgcgtggagactacgcgtgatccgttgaactgggtggcggcggcggagtcgctgaaggggagccacctggacgaggtgaagaggatggtggaggagttcaggaagccggcggtgaagctcggcggtgagagcctgactatagcgcaggtggcggcgatcgcggcgagggataatgcggtggcggtggagctggcggagacggcgcgtgcgggggttaaggcgagtagcgattgggttatggagagtatgaataaagggactgacagttatggagttacaacgggttttggtgccacgtcacataggaggactaaacaaggtggtgctcttcagaaggagctcattaggttcttgaatgccggaatattcggcaacggcacggaatctaaccacgcgctgccacactccgccacgagagccgccatgctcgtccgaatcaacacgctcctccaaggatattccggcatccgatttgaaatcctagaagccctaacaaaattcctcaaccacaacatcaccccctgtttgcccctccgcggcacgatcaccgcctccggcgacctcgtcccgctatcctacattgccgggcttttaacgggccggcccaactccaaggccgtcggcccaaacggcgaagccctcaacgccggcgaggctttcagcctcgccggcgttagcggcttcttcgagctgcaacccaaagaaggcctcgcgctagtcaacgggacagctgtcgggtccggattggcctcgatcgccctgtacgacgcgaacatcctcgccgtcctgtcggaagtgacgtcagtgattttcgctgaggtcatgaatgggaaacctgaatttacggatcatttgacacataagctgaaacatcaccctggccaaattgaggccgctgctataatggaacacattttagatggtagcgcgtacgttaaggctgctcagaaattgcacgaaaccgatccgttgcaaaaaccgaaacaggatcggtacgcgcttagaacgtcgcctcaatggctcggcccccaaatcgaagttatccgaaccgcgacgaaaatgatcgagcgggaaattaattcggttaacgacacacctctaatcgatgtctcgagaaataaagcgttacatggcggtaacttccagggcacgccaatcggggtatcgatggacaacaccagattggcgatagcagctatcggaaaattgatgttcgctcaattttccgagctggttaatgatttctacaacaatggattgccgtctaatctctctggcggtaggaatccgagcttggattacggtttcaaagggtccgaaatcgcgatggcttcgtattgttcggagcttcaatttttagctaatcctgttaccaatcatgtccaaagtgcagagcaacataaccaagatgtgaactcacttggattgatttcttccagaaagaccgtcgaggctctggatattctaaagctgatgtcatccacatatttaatcgcgctatgccaggccgtcgatttgaggcacttggaggagaatttgaggctttcagttaaaaacaccgttagccaagtggcgaagaggactctgacaatgggtattaatggcgaacttcatccgtcaagattctgcgagaaggatcttctccgtgtggtggaccgcgagtacgtgtttgcatacatcgacgatccgtgcagcgggacctaccccttgatgcagaagttgaggcaagttctcgtggaccacgcgttgaacaacggtgagagtgagaaaaacgtgagcacgtctatttttcaaaagatcgaggcgtttgaggtagagttgaaggcgatcttgcctaaagaggtcgagagtgcacggatcgcgttggagagtggaaatccggcgattggtaacaggattacggaatgcagatcgtatccgttgtacaagtttatcagagaggaacttgggacgaactacttgacgggcgaaaaggtcgtttctccgggggaggaatgtgataaggtgttcacagctttgagcaagggtttgattgttgatcctttgttgaagtgtcttgagggttggaatggtgcacctccccctatctgctagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K847100_sequence
1
ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaa
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z