Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Team Bielefeld-CeBiTec 2015
Date created: 2015-08-20 11:00:00
Date modified: 2015-09-19 02:12:15
Translation enhancing 5-UTR containing g10-L RBS
| Types | DnaRegion |
| Roles | Other sequence_feature |
| Sequences | BBa_K1758100_sequence (Version 1) |
Description
This sequence contains a 5' untranslated region (5'-UTR) and a strong ribosomal binding site from bacteriophage T7, named g10-L. This sequence greatly enhances translation of a following gene. The enhancing effect relies on the regulation of mRNA binding to and release of the ribosome S30 subunit (for details see Olins et al. 1989 and Takahashi et al. 2013).g10-L is a strong RBS in E. coli but also well suited for foreign gene expression (Rangwala et al. 1991)
Notes
We derived this sequence from the literature, searching for the best suited enhancing sequence for in vitro translation. Our main literature sources were Olins et al. 1989, Shin and Noireaux 2010, Zawada et al. 2011 and Takahashi et al. 2013.Source
This part was created by applying Gibson primers with suitable overhangson pSB1C3.| Sequence Annotation | Location | Component / Role(s) |
| g10-L RBS 10 A spacer | 32,39 22,31 | ribosome_entry_site feature/rbs feature/misc sequence_feature |