BBa_K1758100 1 BBa_K1758100 Translation enhancing 5-UTR containing g10-L RBS 2015-08-20T11:00:00Z 2015-09-19T02:12:15Z This part was created by applying Gibson primers with suitable overhangson pSB1C3. This sequence contains a 5' untranslated region (5'-UTR) and a strong ribosomal binding site from bacteriophage T7, named ''g10''-L. This sequence greatly enhances translation of a following gene. The enhancing effect relies on the regulation of mRNA binding to and release of the ribosome S30 subunit (for details see [http://www.ncbi.nlm.nih.gov/pubmed/2676996 Olins et al. 1989] and [http://www.ncbi.nlm.nih.gov/pubmed/23927491 Takahashi et al. 2013]). ''g10''-L is a strong RBS in ''E. coli'' but also well suited for foreign gene expression ([http://www.nature.com/nbt/journal/v9/n5/abs/nbt0591-477.html Rangwala et al. 1991]) false false _2180_ 26563 26563 9 false We derived this sequence from the literature, searching for the best suited enhancing sequence for ''in vitro'' translation. Our main literature sources were [http://www.ncbi.nlm.nih.gov/pubmed/2676996 Olins et al. 1989], [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3161345/ Shin and Noireaux 2010], [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3128707/ Zawada et al. 2011] and [http://www.ncbi.nlm.nih.gov/pubmed/23927491 Takahashi et al. 2013]. false Team Bielefeld-CeBiTec 2015 annotation2436605 1 10 A spacer range2436605 1 22 31 annotation2436604 1 g10-L RBS range2436604 1 32 39 BBa_K1758100_sequence 1 aataattttgtttaactttaaaaaaaaaaaagaaggagaataatct igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z