Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Team Bielefeld-CeBiTec 2015
Date created: 2015-08-16 11:00:00
Date modified: 2015-09-19 02:13:09
Translation enhancing 5-UTR + sfGFP
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K1758101_sequence (Version 1) |
Description
This part includes a translation enhancing sequence, 5'-UTR, in front of sfGFP coding sequence (BBa_I746916). It also contains a double terminator made of B0010 and B0012. The translation enhancing sequence improves the binding of the mRNA to the ribosome (Olins et al. 1989) and is especially helpful in in vitro cell free protein synthesis. The sequence contains a spacer of 10 adenine bases which has been shown to further enhance translation efficiency (Takahashi et al. 2013).Notes
Lentini et al. 2013 showed that the scar which is created by standard biobrick assembly is disadvantageous for in vitro translation when occuring between RBS and start codon. Therefore we changed the sequence between RBS and start codon for other parts.Source
This part was created by shortening BBa_I746909 and simultaneously adding 5'-UTR via Gibson primers.Amplification with Gibson-primers:
| Sequence Annotation | Location | Component / Role(s) |
| sfGFP 5'-UTR with g10 RBS B0010 B0012 stop I746916 | 47,763 1,46 775,854 863,903 761,766 47,763 | CDS feature/cds feature/misc sequence_feature feature/BioBrick engineered_region engineered_region feature/BioBrick feature/stop stop_codon engineered_region feature/BioBrick |