BBa_K1758201 1 BBa_K1758201 His-tagged lac repressor under the control of the T7 promoter 2015-08-27T11:00:00Z 2015-09-18T09:11:15Z Parts from the parts registry are combined and a His-Tag is added through PCR and Gibson Assembly. Uses T7 promotor and a strong rbs (BBa_K525998) and the lac repressor with a His-Tag. For a higher production of lacI we combine the gene for lacI with T7-RBS (Protein expression can be induced via rhamnose). By adding a His-Tag, lacI can be purified via Ni-NTA columns. false false _2180_ 26913 26913 9 false This is a composite part which enables a high protein expression and an easy purification of lacI. false Team Bielefeld-CeBiTec 2015 annotation2440027 1 lacI range2440027 1 39 1127 annotation2440028 1 His-Tag range2440028 1 1128 1151 annotation2440023 1 BBa_K525998 range2440023 1 1 32 annotation2440024 1 T7 promoter range2440024 1 1 20 annotation2440025 1 BBa_B0034 range2440025 1 21 32 annotation2440026 1 RBS range2440026 1 24 27 BBa_K1758201_sequence 1 taatacgactcactatagggaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagcaccatcaccatcaccattaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z