LOCUS       BBa_K1758361            2418 bp    DNA     linear       26-May-2024
DEFINITION  mRFP1 controlled by the GABA-inducible activator GabR
ACCESSION   BBa_K1758361
VERSION     BBa_K1758361.1  
FEATURES             Location/Qualifiers
     RBS             1555..1569
                     /label=RBS-1\Strong
     RBS             1562..1566
                     /label=RBS
     misc_feature    1555..1569
                     /label=BBa_B0030
     RBS             1555..1569
                     /label=BBa_B0030
     CDS             1576..2250
                     /label=mrfp1
     misc_feature    2257..2281
                     /label=Help:Barcodes
     CDS             1576..2281
                     /label=BBa_E1010
     stem_loop       2301..2344
                     /label=stem_loop
     misc_feature    2290..2369
                     /label=BBa_B0010
     terminator      2290..2369
                     /label=BBa_B0010
     stem_loop       2385..2404
                     /label=T7 TE
     misc_feature    2411..2411
                     /label=stop
     polyA_site      2405..2418
                     /label=polya
     misc_feature    2378..2418
                     /label=BBa_B0012
     terminator      2378..2418
                     /label=BBa_B0012
     terminator      2290..2418
                     /label=BBa_B0015
     misc_feature    1555..2418
                     /label=BBa_K516030
ORIGIN
        1 tcaatcacct gtgactggga tttttttatg gccgtacact gctttaaaca agcgctgaac
       61 accttcctga atatcttctt ctttgagacg cgcaaaaccg atgatcaggg ctggacgccc
      121 agtctggcgt ttattttctt tcagattgaa gcgcgacatg ccaaaaattt ccaattgcaa
      181 accggccgca tggctcagaa tgtcctgttc tgtacgacgg gtgtcaaatt ccgtcacgaa
      241 gtgcaggccc gcgtttgccc ctttaaccgt cacttcgcca gagaactcgg cctccagggc
      301 cgtgatcagg cgttcacgtt tttctttgta atgctgtttc attttcttaa tgtgcttctg
      361 atattcacct gattcgataa attcttgcag tgtaagctgc gtaagactgc tacaggtttg
      421 caggtcatag ccacgctgtt tatacgcgcg caacaattcc ggcggcagaa ccatgtaaga
      481 aatacgaaga cccggaagca aacttttaga gaaggtaccc atgtagataa cattctgaaa
      541 gcgatccagg ctctgaagcg ccgggatgga gtccacgtcg taagtgaact cactgtcgta
      601 gtcatcttca atgatataac ggcgcggttc ctctgccgcc caattcagga gctgaatgcg
      661 acgagaaacg ggcataatcg taccagatgg aaactggtgg ctaggggttg tcacaagcac
      721 gtcaggctgt tggcgcgtaa tctctgcgat ggacatgccc ttttcatcta acatgatcgt
      781 tttcacttgt ttaccggcgt ttttcagaag ctgatacatg cggcgataac caggttcttc
      841 cattgcgtat actgcctctt tcggcagcag ttcagttaat aactgcatca gcacctgagt
      901 cccggcgcca atgatcatct gttccggacg gcatttgaca ccgcgggtaa gtgaaattaa
      961 gcgggtgatg gcggcacgta cttcgtagat gccctgcgga tggctcatat ctcctaaagt
     1021 acgatacgaa cggctcgcgg ccttttgttc acagcggaac cacgatttaa taggaaagtg
     1081 atcggtatcc gaagacatat gggagaagct gatccaatcg gactgatcaa tatggatttc
     1141 tttcagatcg tccgggaggg cgaacggagg atgttcctct gcgctgaaca tgtccagttc
     1201 ctccacaaag aatcctttgc gctcaatcgc gtacaggtag ccctctgcaa gcagctgctg
     1261 gtatgcgcta ttcacgctat tgactgacac tttgaggttt tcggccagtt cacgtttact
     1321 cggaactttg gaatgtggca gcaagttgcg ggaaagaatt tcttttttca gtttctgata
     1381 gatctgttgg tagatatagt ccgcctgttc cgagcgatcc agagtaatcg taatgtccat
     1441 tttgtagttt ctccttctga taccatcaaa aagttataat tggtactttt catcatacca
     1501 aagagaagtc agaatgataa gaaaataccg acagggggat attcactact agagattaaa
     1561 gaggagaaat actagatggc ttcctccgaa gacgttatca aagagttcat gcgtttcaaa
     1621 gttcgtatgg aaggttccgt taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt
     1681 cgtccgtacg aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg
     1741 ttcgcttggg acatcctgtc cccgcagttc cagtacggtt ccaaagctta cgttaaacac
     1801 ccggctgaca tcccggacta cctgaaactg tccttcccgg aaggtttcaa atgggaacgt
     1861 gttatgaact tcgaagacgg tggtgttgtt accgttaccc aggactcctc cctgcaagac
     1921 ggtgagttca tctacaaagt taaactgcgt ggtaccaact tcccgtccga cggtccggtt
     1981 atgcagaaaa aaaccatggg ttgggaagct tccaccgaac gtatgtaccc ggaagacggt
     2041 gctctgaaag gtgaaatcaa aatgcgtctg aaactgaaag acggtggtca ctacgacgct
     2101 gaagttaaaa ccacctacat ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc
     2161 gacatcaaac tggacatcac ctcccacaac gaagactaca ccatcgttga acagtacgaa
     2221 cgtgctgaag gtcgtcactc caccggtgct taataacgct gatagtgcta gtgtagatcg
     2281 ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     2341 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
     2401 ggcctttctg cgtttata
//