LOCUS       BBa_K1758377            1863 bp    DNA     linear       20-May-2024
DEFINITION  Biosensor device for detection of GHB and GBL
ACCESSION   BBa_K1758377
VERSION     BBa_K1758377.1  
FEATURES             Location/Qualifiers
     promoter        1..22
                     /label=T7 promoter
     misc_feature    23..63
                     /label=Blc operator
     misc_feature    64..109
                     /label=5' -UTR
     misc_feature    64..109
                     /label=K1758100
     misc_feature    64..966
                     /label=K1758101
     CDS             110..829
                     /label=sfGFP
     misc_feature    838..917
                     /label=B0010
     misc_feature    926..966
                     /label=B0012
     misc_feature    691..691
                     /label=silent mutation
     misc_feature    1..966
                     /label=BBa_K1758376
     misc_feature    975..1029
                     /label=K608002
     CDS             1036..1863
                     /label=BlcR
     misc_feature    975..1863
                     /label=BBa_K1758370
ORIGIN
        1 taatacgact cactataggg agttcactct aatgattcaa gttcaattag ttgaactcta
       61 atgaataatt ttgtttaact ttaaaaaaaa aaaagaagga gaataatcta tgcgtaaagg
      121 cgaagagctg ttcactggtg tcgtccctat tctggtggaa ctggatggtg atgtcaacgg
      181 tcataagttt tccgtgcgtg gcgagggtga aggtgacgca actaatggta aactgacgct
      241 gaagttcatc tgtactactg gtaaactgcc ggtaccttgg ccgactctgg taacgacgct
      301 gacttatggt gttcagtgct ttgctcgtta tccggaccat atgaagcagc atgacttctt
      361 caagtccgcc atgccggaag gctatgtgca ggaacgcacg atttccttta aggatgacgg
      421 cacgtacaaa acgcgtgcgg aagtgaaatt tgaaggcgat accctggtaa accgcattga
      481 gctgaaaggc attgacttta aagaagacgg caatatcctg ggccataagc tggaatacaa
      541 ttttaacagc cacaatgttt acatcaccgc cgataaacaa aaaaatggca ttaaagcgaa
      601 ttttaaaatt cgccacaacg tggaggatgg cagcgtgcag ctggctgatc actaccagca
      661 aaacactcca atcggtgatg gtcctgttct actgccagac aatcactatc tgagcacgca
      721 aagcgttctg tctaaagatc cgaacgagaa acgcgatcat atggttctgc tggagttcgt
      781 aaccgcagcg ggcatcacgc atggtatgga tgaactgtac aaatgatgat actagagcca
      841 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
      901 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
      961 tttatatact agagttgaca gctagctcag tcctaggtat tgtgctagct actagagaaa
     1021 gaggagaaat actagatggg ccaaaggggc caagtgtgtc aaggaaggtg catggctgaa
     1081 gatcaacaat cgtcgcaaat atcagacact gtaccagcac tcagacgcgc cgtgcgtatt
     1141 ctggatcttg tggcaggttc cccgcgggac cttacagccg ccgagctgac acgcattctg
     1201 gatttgccca aaagcagcgc gcatggcttg cttgcggtga tgactgagct tgatcttctg
     1261 gcgcgatctg ccgatggaac cctgcgtatt ggaccccact cgctcagatg ggcaaatggt
     1321 tttctgtcgc acctcgatat agtatcgaca ttcaacgacc atctcgccca gcgccacgac
     1381 ctcgatccct acacggtgac cctcaccgtc cgcgagggtg gcgaagtcgt ttacatcggc
     1441 tgtcgcaact cggctcagcc gcttggacac acgttccgga tcggtatgcg tctgccggcg
     1501 ccatttacgg ccaccgggaa gattctcctg tccgatctgg ggcctggtga attgaggatg
     1561 ctgttctctc agtttccaca gcctctgaca tcaaggagtg ttgctggcct ttcgcagctt
     1621 gaagaggaac tggctctgac gcgcgctcgc ggctactcca tcgacgatgg tcagatccgc
     1681 gaaggtatgc tttgcattgg ggctgcgata cgcgattact cgggagccgc atctgccggc
     1741 attgcaatca gtctgatccg cagcgaagcc agcgacgaaa aaatcgctag ccttggtgag
     1801 gagcttcgca ccactgccaa cgcgctttct gaaaagcttg ggtaccgatc gcagaaagac
     1861 taa
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