LOCUS       BBa_K1758390            1474 bp    DNA     linear       15-May-2024
DEFINITION  His-tagged E. coli tryptophanase under control of the T7 promoter
ACCESSION   BBa_K1758390
VERSION     BBa_K1758390.1  
FEATURES             Location/Qualifiers
     misc_feature    1454..1471
                     /label=his-tag
     misc_feature    1472..1474
                     /label=stop
     CDS             41..1453
                     /label=tryptophanase
     promoter        1..32
                     /label=T7 promoter + RBS
ORIGIN
        1 taatacgact cactataggg aaagaggaga aatactagag atggagaatt tcaaacactt
       61 accggagccc ttccgcattc gcgtcatcga gccggtaaag cgtacaacgc gtgcgtatcg
      121 tgaagaagcg attatcaaaa gtggaatgaa cccatttctg ctggatagcg aagatgtgtt
      181 tatcgatctt ctgaccgata gtggcaccgg cgcggttaca caatcaatgc aggctgcgat
      241 gatgcgcggc gatgaagctt actcgggctc tcgtagctat tacgccttag cagagagcgt
      301 gaaaaatatc ttcggctacc aatataccat tccaacccat cagggacgtg gtgccgagca
      361 aatttacatc ccagtgctga ttaaaaaacg cgaacaggaa aaaggtttag atcgctccaa
      421 aatggttgcc ttctcaaact attttttcga cacaacacag gggcactccc agatcaacgg
      481 ttgcacggtg cgcaacgtct atattaaaga ggcgtttgat acgggcgtcc gctatgattt
      541 taaaggcaat ttcgatttgg aaggcctgga gcgtggcatc gaggaagtgg gcccgaataa
      601 tgttccatat attgtcgcaa ccattaccag caacagtgcc ggcggtcaac ctgttagcct
      661 ggcgaatctg aaggccatgt atagtatcgc caagaaatat gacatcccgg tggtgatgga
      721 tagtgcgcgt ttcgcagaga atgcctactt catcaaacag cgcgaagctg aatacaagga
      781 ttggacaatt gaacaaatca cgcgtgaaac ttacaaatac gcggacatgc tcgcgatgtc
      841 agcgaaaaag gatgcaatgg tccctatggg tggtctgctg tgcatgaaag atgacagttt
      901 tttcgacgtt tatacagaat gccgtacact gtgcgttgtg caggaaggtt tccctacata
      961 tggtggtctg gaaggaggtg caatggaacg tctggcagta ggcctgtatg acggcatgaa
     1021 ccttgactgg ctggcctatc gtattgccca ggtccagtat ctggtcgacg gactggaaga
     1081 aatcggcgta gtctgccagc aggcgggcgg ccatgcggcc tttgttgacg ctggcaaact
     1141 tttgcctcat atcccggcag atcagttccc cgctcaggcc ttagcgtgcg agctgtataa
     1201 agttgcaggg atccgtgcgg tcgaaatcgg ctctttcttg ttgggacgtg atccgaaaac
     1261 cggtaaacaa ttgccatgcc cggctgaact gctgcgcttg accattccac gtgccaccta
     1321 tacgcaaacg cacatggatt ttattatcga agctttcaaa catgtgaaag aaaatgcggc
     1381 gaacattaaa ggcctgactt ttacatatga accgaaagta ctgcgtcact ttaccgccaa
     1441 actgaaagaa gttcatcatc accatcacca ctaa
//