LOCUS BBa_K176030 2790 bp DNA linear 07-Jun-2024 DEFINITION Repressible AHL->GFP Receiver: PoPS->tetR-LVA+pCon 0.70->luxR+pLux/Tet->GFP ACCESSION BBa_K176030 VERSION BBa_K176030.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 CDS 22..638 /label=tetR misc_feature 639..672 /label=SsrA misc_feature 679..703 /label=Help:Barcodes CDS 19..678 /label=BBa_C0040 misc_feature 1..703 /label=BBa_S0101 stem_loop 723..766 /label=stem_loop misc_feature 712..791 /label=BBa_B0010 terminator 712..791 /label=BBa_B0010 stem_loop 807..826 /label=T7 TE misc_feature 833..833 /label=stop polyA_site 827..840 /label=polya misc_feature 800..840 /label=BBa_B0012 terminator 800..840 /label=BBa_B0012 terminator 712..840 /label=BBa_B0015 misc_feature 1..840 /label=BBa_K176006 promoter 849..883 /label=BBa_J23101 misc_feature 896..899 /label=conserved RBS 892..903 /label=BBa_B0034 misc_feature 910..911 /label=prefix misc_feature 1083..1083 /label=T misc_feature 1401..1401 /label=A CDS 910..1659 /label=luxR misc_feature 910..1665 /label=BBa_C0062 misc_feature 1666..1690 /label=Help:Barcodes CDS 910..1665 /label=BBa_C0062 stem_loop 1710..1753 /label=stem_loop misc_feature 1699..1778 /label=BBa_B0010 terminator 1699..1778 /label=BBa_B0010 stem_loop 1794..1813 /label=T7 TE misc_feature 1820..1820 /label=stop polyA_site 1814..1827 /label=polya misc_feature 1787..1827 /label=BBa_B0012 terminator 1787..1827 /label=BBa_B0012 misc_feature 892..1827 /label=BBa_I0462 misc_feature 849..1827 /label=BBa_K176025 misc_feature 1836..1888 /label=R0062 promoter 1855..1860 /label=-35 promoter 1877..1882 /label=-10 misc_feature 1889..1907 /label=tetR misc_feature 1888..1888 /label=+1 misc_feature 1836..1855 /label=luxR/HSL promoter 1836..1907 /label=BBa_K176000 misc_feature 1920..1923 /label=conserved RBS 1916..1927 /label=BBa_B0034 CDS 1934..2653 /label=GFP protein CDS 1934..2653 /label=BBa_E0040 stem_loop 2673..2716 /label=stem_loop misc_feature 2662..2741 /label=BBa_B0010 terminator 2662..2741 /label=BBa_B0010 stem_loop 2757..2776 /label=T7 TE misc_feature 2783..2783 /label=stop polyA_site 2777..2790 /label=polya misc_feature 2750..2790 /label=BBa_B0012 terminator 2750..2790 /label=BBa_B0012 misc_feature 1836..2790 /label=BBa_K176002 misc_feature 849..2790 /label=BBa_K176026 ORIGIN 1 aaagaggaga aatactagat gtccagatta gataaaagta aagtgattaa cagcgcatta 61 gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta 121 ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgggcttt gctcgacgcc 181 ttagccattg agatgttaga taggcaccat actcactttt gccctttaga aggggaaagc 241 tggcaagatt ttttacgtaa taacgctaaa agttttagat gtgctttact aagtcatcgc 301 gatggagcaa aagtacattt aggtacacgg cctacagaaa aacagtatga aactctcgaa 361 aatcaattag cctttttatg ccaacaaggt ttttcactag agaatgcatt atatgcactc 421 agcgctgtgg ggcattttac tttaggttgc gtattggaag atcaagagca tcaagtcgct 481 aaagaagaaa gggaaacacc tactactgat agtatgccgc cattattacg acaagctatc 541 gaattatttg atcaccaagg tgcagagcca gccttcttat tcggccttga attgatcata 601 tgcggattag aaaaacaact taaatgtgaa agtgggtccg ctgcaaacga cgaaaactac 661 gctttagtag cttaataaca ctgatagtgc tagtgtagat cactactaga gccaggcatc 721 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 781 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata 841 tactagagtt tacagctagc tcagtcctag gtattatgct agctactaga gaaagaggag 901 aaatactaga tgaaaaacat aaatgccgac gacacataca gaataattaa taaaattaaa 961 gcttgtagaa gcaataatga tattaatcaa tgcttatctg atatgactaa aatggtacat 1021 tgtgaatatt atttactcgc gatcatttat cctcattcta tggttaaatc tgatatttca 1081 atcctagata attaccctaa aaaatggagg caatattatg atgacgctaa tttaataaaa 1141 tatgatccta tagtagatta ttctaactcc aatcattcac caattaattg gaatatattt 1201 gaaaacaatg ctgtaaataa aaaatctcca aatgtaatta aagaagcgaa aacatcaggt 1261 cttatcactg ggtttagttt ccctattcat acggctaaca atggcttcgg aatgcttagt 1321 tttgcacatt cagaaaaaga caactatata gatagtttat ttttacatgc gtgtatgaac 1381 ataccattaa ttgttccttc tctagttgat aattatcgaa aaataaatat agcaaataat 1441 aaatcaaaca acgatttaac caaaagagaa aaagaatgtt tagcgtgggc atgcgaagga 1501 aaaagctctt gggatatttc aaaaatatta ggttgcagtg agcgtactgt cactttccat 1561 ttaaccaatg cgcaaatgaa actcaataca acaaaccgct gccaaagtat ttctaaagca 1621 attttaacag gagcaattga ttgcccatac tttaaaaatt aataacactg atagtgctag 1681 tgtagatcac tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg 1741 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac 1801 cttcgggtgg gcctttctgc gtttatatac tagagacctg taggatcgta caggtttacg 1861 caagaaaatg gtttgttata gtcgaatatc cctatcagtg atagagatac tagagaaaga 1921 ggagaaatac tagatgcgta aaggagaaga acttttcact ggagttgtcc caattcttgt 1981 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga 2041 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc 2101 atggccaaca cttgtcacta ctttcggtta tggtgttcaa tgctttgcga gatacccaga 2161 tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag 2221 aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg 2281 tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat 2341 tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa 2401 acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt 2461 tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc 2521 agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga 2581 ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact 2641 atacaaataa taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact 2701 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct 2761 caccttcggg tgggcctttc tgcgtttata //