LOCUS       BBa_K176131             1530 bp    DNA     linear       17-May-2024
DEFINITION  TetR repressible AHL->Death Receiver: pCon 0.36->luxR+pLux/Tet->ccd
            B(weak RBS)
ACCESSION   BBa_K176131
VERSION     BBa_K176131.1  
FEATURES             Location/Qualifiers
     promoter        1..6
                     /label=-35
     misc_feature    7..12
                     /label=NheI
     misc_feature    17..17
                     /label=c in J23107
     misc_feature    18..23
                     /label=AvrII
     promoter        24..29
                     /label=-10
     misc_feature    30..35
                     /label=NheI
     promoter        1..35
                     /label=BBa_K176009
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    62..63
                     /label=prefix
     misc_feature    235..235
                     /label=T
     misc_feature    553..553
                     /label=A
     CDS             62..811
                     /label=luxR
     misc_feature    62..817
                     /label=BBa_C0062
     misc_feature    818..842
                     /label=Help:Barcodes
     CDS             62..817
                     /label=BBa_C0062
     stem_loop       862..905
                     /label=stem_loop
     misc_feature    851..930
                     /label=BBa_B0010
     terminator      851..930
                     /label=BBa_B0010
     stem_loop       946..965
                     /label=T7 TE
     misc_feature    972..972
                     /label=stop
     polyA_site      966..979
                     /label=polya
     misc_feature    939..979
                     /label=BBa_B0012
     terminator      939..979
                     /label=BBa_B0012
     misc_feature    44..979
                     /label=BBa_I0462
     misc_feature    988..1040
                     /label=R0062
     promoter        1007..1012
                     /label=-35
     promoter        1029..1034
                     /label=-10
     misc_feature    1041..1059
                     /label=tetR
     misc_feature    1040..1040
                     /label=+1
     misc_feature    988..1007
                     /label=luxR/HSL
     promoter        988..1059
                     /label=BBa_K176000
     misc_feature    44..1059
                     /label=BBa_K176123
     misc_feature    1..1059
                     /label=BBa_K176125
     misc_feature    1074..1077
                     /label=conserved
     RBS             1068..1081
                     /label=BBa_B0031
     misc_feature    1088..1090
                     /label=start
     misc_feature    1391..1393
                     /label=stop
     CDS             1088..1390
                     /label=cds
     CDS             1088..1393
                     /label=BBa_K145151
     stem_loop       1413..1456
                     /label=stem_loop
     misc_feature    1402..1481
                     /label=BBa_B0010
     terminator      1402..1481
                     /label=BBa_B0010
     stem_loop       1497..1516
                     /label=T7 TE
     misc_feature    1523..1523
                     /label=stop
     polyA_site      1517..1530
                     /label=polya
     misc_feature    1490..1530
                     /label=BBa_B0012
     terminator      1490..1530
                     /label=BBa_B0012
     misc_feature    1068..1530
                     /label=BBa_K176062
ORIGIN
        1 tttacggcta gctcagtcct aggtattatg ctagctacta gagaaagagg agaaatacta
       61 gatgaaaaac ataaatgccg acgacacata cagaataatt aataaaatta aagcttgtag
      121 aagcaataat gatattaatc aatgcttatc tgatatgact aaaatggtac attgtgaata
      181 ttatttactc gcgatcattt atcctcattc tatggttaaa tctgatattt caatcctaga
      241 taattaccct aaaaaatgga ggcaatatta tgatgacgct aatttaataa aatatgatcc
      301 tatagtagat tattctaact ccaatcattc accaattaat tggaatatat ttgaaaacaa
      361 tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg aaaacatcag gtcttatcac
      421 tgggtttagt ttccctattc atacggctaa caatggcttc ggaatgctta gttttgcaca
      481 ttcagaaaaa gacaactata tagatagttt atttttacat gcgtgtatga acataccatt
      541 aattgttcct tctctagttg ataattatcg aaaaataaat atagcaaata ataaatcaaa
      601 caacgattta accaaaagag aaaaagaatg tttagcgtgg gcatgcgaag gaaaaagctc
      661 ttgggatatt tcaaaaatat taggttgcag tgagcgtact gtcactttcc atttaaccaa
      721 tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt atttctaaag caattttaac
      781 aggagcaatt gattgcccat actttaaaaa ttaataacac tgatagtgct agtgtagatc
      841 actactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
      901 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
      961 gggcctttct gcgtttatat actagagacc tgtaggatcg tacaggttta cgcaagaaaa
     1021 tggtttgtta tagtcgaata tccctatcag tgatagagat actagagtca cacaggaaac
     1081 ctactagatg cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt
     1141 ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc ccctggccag
     1201 tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc atatcgggga
     1261 tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg ttatcgggga
     1321 agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt
     1381 ctggggaata taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
     1441 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
     1501 caccttcggg tgggcctttc tgcgtttata
//