LOCUS       BBa_K176168             1735 bp    DNA     linear       17-May-2024
DEFINITION  TetR repressible AHL->Death Receiver: pCon 0.70->luxR+pLux/Tet->lac
            Zalpha-ccdB-LVA
ACCESSION   BBa_K176168
VERSION     BBa_K176168.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    62..63
                     /label=prefix
     misc_feature    235..235
                     /label=T
     misc_feature    553..553
                     /label=A
     CDS             62..811
                     /label=luxR
     misc_feature    62..817
                     /label=BBa_C0062
     misc_feature    818..842
                     /label=Help:Barcodes
     CDS             62..817
                     /label=BBa_C0062
     stem_loop       862..905
                     /label=stem_loop
     misc_feature    851..930
                     /label=BBa_B0010
     terminator      851..930
                     /label=BBa_B0010
     stem_loop       946..965
                     /label=T7 TE
     misc_feature    972..972
                     /label=stop
     polyA_site      966..979
                     /label=polya
     misc_feature    939..979
                     /label=BBa_B0012
     terminator      939..979
                     /label=BBa_B0012
     misc_feature    44..979
                     /label=BBa_I0462
     misc_feature    1..979
                     /label=BBa_K176025
     misc_feature    988..1040
                     /label=R0062
     promoter        1007..1012
                     /label=-35
     promoter        1029..1034
                     /label=-10
     misc_feature    1041..1059
                     /label=tetR
     misc_feature    1040..1040
                     /label=+1
     misc_feature    988..1007
                     /label=luxR/HSL
     promoter        988..1059
                     /label=BBa_K176000
     misc_feature    1..1059
                     /label=BBa_K176028
     misc_feature    1072..1075
                     /label=conserved
     RBS             1068..1079
                     /label=BBa_B0034
     CDS             1086..1559
                     /label=lacZalpha-ccdB
     misc_feature    1560..1592
                     /label=LVA degradation tag (AANDENYALVA)
     misc_feature    1593..1598
                     /label=Stop
     misc_feature    1086..1250
                     /label=a part of lacZalpha (I732006)
     misc_feature    1086..1088
                     /label=Start
     misc_feature    1101..1101
                     /label=177bp and a in J32026
     primer_bind     1106..1122
                     /label=M13 -20 primer
     primer_bind     1126..1149
                     /label=M13 -47 primer (K176059)
     primer_bind     1126..1142
                     /label=M13 -40 primer
     misc_feature    1250..1250
                     /label=g and 69bp in I732006
     misc_feature    1257..1258
                     /label=at in K145151
     misc_feature    1257..1559
                     /label=ccdB (K145151)
     primer_bind     1273..1300
                     /label=VRccdB primer (K176058)
     misc_feature    1340..1340
                     /label=c in K145151
     CDS             1086..1598
                     /label=BBa_K176158
     stem_loop       1618..1661
                     /label=stem_loop
     misc_feature    1607..1686
                     /label=BBa_B0010
     terminator      1607..1686
                     /label=BBa_B0010
     stem_loop       1702..1721
                     /label=T7 TE
     misc_feature    1728..1728
                     /label=stop
     polyA_site      1722..1735
                     /label=polya
     misc_feature    1695..1735
                     /label=BBa_B0012
     terminator      1695..1735
                     /label=BBa_B0012
     misc_feature    1068..1735
                     /label=BBa_K176164
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagaaagagg agaaatacta
       61 gatgaaaaac ataaatgccg acgacacata cagaataatt aataaaatta aagcttgtag
      121 aagcaataat gatattaatc aatgcttatc tgatatgact aaaatggtac attgtgaata
      181 ttatttactc gcgatcattt atcctcattc tatggttaaa tctgatattt caatcctaga
      241 taattaccct aaaaaatgga ggcaatatta tgatgacgct aatttaataa aatatgatcc
      301 tatagtagat tattctaact ccaatcattc accaattaat tggaatatat ttgaaaacaa
      361 tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg aaaacatcag gtcttatcac
      421 tgggtttagt ttccctattc atacggctaa caatggcttc ggaatgctta gttttgcaca
      481 ttcagaaaaa gacaactata tagatagttt atttttacat gcgtgtatga acataccatt
      541 aattgttcct tctctagttg ataattatcg aaaaataaat atagcaaata ataaatcaaa
      601 caacgattta accaaaagag aaaaagaatg tttagcgtgg gcatgcgaag gaaaaagctc
      661 ttgggatatt tcaaaaatat taggttgcag tgagcgtact gtcactttcc atttaaccaa
      721 tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt atttctaaag caattttaac
      781 aggagcaatt gattgcccat actttaaaaa ttaataacac tgatagtgct agtgtagatc
      841 actactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
      901 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
      961 gggcctttct gcgtttatat actagagacc tgtaggatcg tacaggttta cgcaagaaaa
     1021 tggtttgtta tagtcgaata tccctatcag tgatagagat actagagaaa gaggagaaat
     1081 actagatgac catgattacg gattcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     1141 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     1201 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagccta tacgtacggc
     1261 agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga
     1321 gtgatattat tgacacgccg gggcgacgga tggtgatccc cctggccagt gcacgtctgc
     1381 tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat gaaagctggc
     1441 gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa gaagtggctg
     1501 atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatag
     1561 ctgcaaacga cgaaaactac gctttagtag cttaataata ctagagccag gcatcaaata
     1621 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     1681 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//