LOCUS       BBa_K176189             1656 bp    DNA     linear       13-May-2024
DEFINITION  CI Inverter + luxI-LVA: PoPS->cI-LVA+pCI->luxI-LVA->PoPS
ACCESSION   BBa_K176189
VERSION     BBa_K176189.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             22..729
                     /label=cI lambda
     misc_feature    730..762
                     /label=LVA
     misc_feature    769..793
                     /label=Help:Barcodes
     CDS             19..768
                     /label=BBa_C0051
     stem_loop       813..856
                     /label=stem_loop
     misc_feature    802..881
                     /label=BBa_B0010
     terminator      802..881
                     /label=BBa_B0010
     stem_loop       897..916
                     /label=T7 TE
     misc_feature    923..923
                     /label=stop
     polyA_site      917..930
                     /label=polya
     misc_feature    890..930
                     /label=BBa_B0012
     terminator      890..930
                     /label=BBa_B0012
     promoter        976..981
                     /label=-10
     promoter        953..958
                     /label=-35
     misc_feature    963..979
                     /label=OR1
     misc_feature    939..955
                     /label=OR2
     misc_feature    939..987
                     /label=BBa_R0051
     promoter        939..987
                     /label=BBa_R0051
     misc_feature    1..987
                     /label=BBa_Q04510
     misc_feature    1000..1003
                     /label=conserved
     RBS             996..1007
                     /label=BBa_B0034
     misc_feature    1593..1624
                     /label=LVA
     CDS             1014..1592
                     /label=luxI
     misc_feature    1014..1631
                     /label=BBa_C0061
     misc_feature    1632..1656
                     /label=Help:Barcodes
     CDS             1014..1631
                     /label=BBa_C0061
     misc_feature    996..1656
                     /label=BBa_C0261
ORIGIN
        1 aaagaggaga aatactagat gagcacaaaa aagaaaccat taacacaaga gcagcttgag
       61 gacgcacgtc gccttaaagc aatttatgaa aaaaagaaaa atgaacttgg cttatcccag
      121 gaatctgtcg cagacaagat ggggatgggg cagtcaggcg ttggtgcttt atttaatggc
      181 atcaatgcat taaatgctta taacgccgca ttgcttgcaa aaattctcaa agttagcgtt
      241 gaagaattta gcccttcaat cgccagagaa atctacgaga tgtatgaagc ggttagtatg
      301 cagccgtcac ttagaagtga gtatgagtac cctgtttttt ctcatgttca ggcagggatg
      361 ttctcacctg agcttagaac ctttaccaaa ggtgatgcgg agagatgggt aagcacaacc
      421 aaaaaagcca gtgattctgc attctggctt gaggttgaag gtaattccat gaccgcacca
      481 acaggctcca agccaagctt tcctgacgga atgttaattc tcgttgaccc tgagcaggct
      541 gttgagccag gtgatttctg catagccaga cttgggggtg atgagtttac cttcaagaaa
      601 ctgatcaggg atagcggtca ggtgttttta caaccactaa acccacagta cccaatgatc
      661 ccatgcaatg agagttgttc cgttgtgggg aaagttatcg ctagtcagtg gcctgaagag
      721 acgtttggcg ctgcaaacga cgaaaactac gctttagtag cttaataacg ctgatagtgc
      781 tagtgtagat cgctactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
      841 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
      901 caccttcggg tgggcctttc tgcgtttata tactagagta acaccgtgcg tgttgactat
      961 tttacctctg gcggtgataa tggttgctac tagagaaaga ggagaaatac tagatgacta
     1021 taatgataaa aaaatcggat tttttggcaa ttccatcgga ggagtataaa ggtattctaa
     1081 gtcttcgtta tcaagtgttt aagcaaagac ttgagtggga cttagttgta gaaaataacc
     1141 ttgaatcaga tgagtatgat aactcaaatg cagaatatat ttatgcttgt gatgatactg
     1201 aaaatgtaag tggatgctgg cgtttattac ctacaacagg tgattatatg ctgaaaagtg
     1261 tttttcctga attgcttggt caacagagtg ctcccaaaga tcctaatata gtcgaattaa
     1321 gtcgttttgc tgtaggtaaa aatagctcaa agataaataa ctctgctagt gaaattacaa
     1381 tgaaactatt tgaagctata tataaacacg ctgttagtca aggtattaca gaatatgtaa
     1441 cagtaacatc aacagcaata gagcgatttt taaagcgtat taaagttcct tgtcatcgta
     1501 ttggagacaa agaaattcat gtattaggtg atactaaatc ggttgtattg tctatgccta
     1561 ttaatgaaca gtttaaaaaa gcagtcttaa atgctgcaaa cgacgaaaac tacgctttag
     1621 tagcttaata actctgatag tgctagtgta gatctc
//