LOCUS BBa_K176219 2791 bp DNA linear 17-May-2024 DEFINITION cell density->GFP: pCon 0.15->luxI-LVA+pCon 0.70->luxR+pLux/Tet->GF P ACCESSION BBa_K176219 VERSION BBa_K176219.1 FEATURES Location/Qualifiers promoter 1..6 /label=-35 promoter 24..29 /label=-10 misc_feature 17..17 /label=c in J23115 misc_feature 21..21 /label=t in J23115 misc_feature 7..12 /label=NheI misc_feature 30..35 /label=NheI misc_feature 18..23 /label=AvrII promoter 1..35 /label=BBa_K176008 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 misc_feature 641..672 /label=LVA CDS 62..640 /label=luxI misc_feature 62..679 /label=BBa_C0061 misc_feature 680..704 /label=Help:Barcodes CDS 62..679 /label=BBa_C0061 misc_feature 44..704 /label=BBa_C0261 stem_loop 724..767 /label=stem_loop misc_feature 713..792 /label=BBa_B0010 terminator 713..792 /label=BBa_B0010 stem_loop 808..827 /label=T7 TE misc_feature 834..834 /label=stop polyA_site 828..841 /label=polya misc_feature 801..841 /label=BBa_B0012 terminator 801..841 /label=BBa_B0012 terminator 713..841 /label=BBa_B0015 misc_feature 44..841 /label=BBa_K082014 misc_feature 1..841 /label=BBa_K176022 promoter 850..884 /label=BBa_J23101 misc_feature 897..900 /label=conserved RBS 893..904 /label=BBa_B0034 misc_feature 911..912 /label=prefix misc_feature 1084..1084 /label=T misc_feature 1402..1402 /label=A CDS 911..1660 /label=luxR misc_feature 911..1666 /label=BBa_C0062 misc_feature 1667..1691 /label=Help:Barcodes CDS 911..1666 /label=BBa_C0062 stem_loop 1711..1754 /label=stem_loop misc_feature 1700..1779 /label=BBa_B0010 terminator 1700..1779 /label=BBa_B0010 stem_loop 1795..1814 /label=T7 TE misc_feature 1821..1821 /label=stop polyA_site 1815..1828 /label=polya misc_feature 1788..1828 /label=BBa_B0012 terminator 1788..1828 /label=BBa_B0012 misc_feature 893..1828 /label=BBa_I0462 misc_feature 850..1828 /label=BBa_K176025 misc_feature 1837..1889 /label=R0062 promoter 1856..1861 /label=-35 promoter 1878..1883 /label=-10 misc_feature 1890..1908 /label=tetR misc_feature 1889..1889 /label=+1 misc_feature 1837..1856 /label=luxR/HSL promoter 1837..1908 /label=BBa_K176000 misc_feature 850..1908 /label=BBa_K176028 misc_feature 1..1908 /label=BBa_K176085 misc_feature 1921..1924 /label=conserved RBS 1917..1928 /label=BBa_B0034 CDS 1935..2654 /label=GFP protein CDS 1935..2654 /label=BBa_E0040 stem_loop 2674..2717 /label=stem_loop misc_feature 2663..2742 /label=BBa_B0010 terminator 2663..2742 /label=BBa_B0010 stem_loop 2758..2777 /label=T7 TE misc_feature 2784..2784 /label=stop polyA_site 2778..2791 /label=polya misc_feature 2751..2791 /label=BBa_B0012 terminator 2751..2791 /label=BBa_B0012 misc_feature 1917..2791 /label=BBa_I13504 ORIGIN 1 tttatagcta gctcagtcct aggtacaatg ctagctacta gagaaagagg agaaatacta 61 gatgactata atgataaaaa aatcggattt tttggcaatt ccatcggagg agtataaagg 121 tattctaagt cttcgttatc aagtgtttaa gcaaagactt gagtgggact tagttgtaga 181 aaataacctt gaatcagatg agtatgataa ctcaaatgca gaatatattt atgcttgtga 241 tgatactgaa aatgtaagtg gatgctggcg tttattacct acaacaggtg attatatgct 301 gaaaagtgtt tttcctgaat tgcttggtca acagagtgct cccaaagatc ctaatatagt 361 cgaattaagt cgttttgctg taggtaaaaa tagctcaaag ataaataact ctgctagtga 421 aattacaatg aaactatttg aagctatata taaacacgct gttagtcaag gtattacaga 481 atatgtaaca gtaacatcaa cagcaataga gcgattttta aagcgtatta aagttccttg 541 tcatcgtatt ggagacaaag aaattcatgt attaggtgat actaaatcgg ttgtattgtc 601 tatgcctatt aatgaacagt ttaaaaaagc agtcttaaat gctgcaaacg acgaaaacta 661 cgctttagta gcttaataac tctgatagtg ctagtgtaga tctctactag agccaggcat 721 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 781 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 841 atactagagt ttacagctag ctcagtccta ggtattatgc tagctactag agaaagagga 901 gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta ataaaattaa 961 agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta aaatggtaca 1021 ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat ctgatatttc 1081 aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta atttaataaa 1141 atatgatcct atagtagatt attctaactc caatcattca ccaattaatt ggaatatatt 1201 tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga aaacatcagg 1261 tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg gaatgcttag 1321 ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg cgtgtatgaa 1381 cataccatta attgttcctt ctctagttga taattatcga aaaataaata tagcaaataa 1441 taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg catgcgaagg 1501 aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg tcactttcca 1561 tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta tttctaaagc 1621 aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact gatagtgcta 1681 gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg 1741 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca 1801 ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt acaggtttac 1861 gcaagaaaat ggtttgttat agtcgaatat ccctatcagt gatagagata ctagagaaag 1921 aggagaaata ctagatgcgt aaaggagaag aacttttcac tggagttgtc ccaattcttg 1981 ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg 2041 atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc 2101 catggccaac acttgtcact actttcggtt atggtgttca atgctttgcg agatacccag 2161 atcatatgaa acagcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa 2221 gaactatatt tttcaaagat gacgggaact acaagacacg tgctgaagtc aagtttgaag 2281 gtgataccct tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca 2341 ttcttggaca caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca 2401 aacaaaagaa tggaatcaaa gttaacttca aaattagaca caacattgaa gatggaagcg 2461 ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac 2521 cagacaacca ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag 2581 accacatggt ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac 2641 tatacaaata ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac 2701 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc 2761 tcaccttcgg gtgggccttt ctgcgtttat a //