LOCUS BBa_K176244 3496 bp DNA linear 17-May-2024 DEFINITION For characterization of K176240: VR9+pCon 0.15->K176240->PoPS ACCESSION BBa_K176244 VERSION BBa_K176244.1 FEATURES Location/Qualifiers primer_bind 1..20 /label=VR9 misc_feature 1..20 /label=BBa_K176043 promoter 29..34 /label=-35 promoter 52..57 /label=-10 misc_feature 45..45 /label=c in J23115 misc_feature 49..49 /label=t in J23115 misc_feature 35..40 /label=NheI misc_feature 58..63 /label=NheI misc_feature 46..51 /label=AvrII promoter 29..63 /label=BBa_K176008 promoter 1..63 /label=BBa_K176103 misc_feature 76..79 /label=conserved RBS 72..83 /label=BBa_B0034 misc_feature 669..700 /label=LVA CDS 90..668 /label=luxI misc_feature 90..707 /label=BBa_C0061 misc_feature 708..732 /label=Help:Barcodes CDS 90..707 /label=BBa_C0061 misc_feature 72..732 /label=BBa_C0261 misc_feature 1636..1639 /label=conserved RBS 1632..1643 /label=BBa_B0034 misc_feature 2229..2260 /label=LVA CDS 1650..2228 /label=luxI misc_feature 1650..2267 /label=BBa_C0061 misc_feature 2268..2292 /label=Help:Barcodes CDS 1650..2267 /label=BBa_C0061 misc_feature 1632..2292 /label=BBa_C0261 misc_feature 745..748 /label=conserved RBS 741..752 /label=BBa_B0034 CDS 762..1378 /label=tetR misc_feature 1379..1412 /label=SsrA misc_feature 1419..1443 /label=Help:Barcodes CDS 759..1418 /label=BBa_C0040 misc_feature 741..1443 /label=BBa_S0101 stem_loop 1463..1506 /label=stem_loop misc_feature 1452..1531 /label=BBa_B0010 terminator 1452..1531 /label=BBa_B0010 stem_loop 1547..1566 /label=T7 TE misc_feature 1573..1573 /label=stop polyA_site 1567..1580 /label=polya misc_feature 1540..1580 /label=BBa_B0012 terminator 1540..1580 /label=BBa_B0012 terminator 1452..1580 /label=BBa_B0015 misc_feature 741..1580 /label=BBa_K176006 promoter 1589..1594 /label=-35 promoter 1612..1617 /label=-10 misc_feature 1605..1605 /label=c in J23115 misc_feature 1609..1609 /label=t in J23115 misc_feature 1595..1600 /label=NheI misc_feature 1618..1623 /label=NheI misc_feature 1606..1611 /label=AvrII promoter 1589..1623 /label=BBa_K176008 misc_feature 1636..1639 /label=conserved RBS 1632..1643 /label=BBa_B0034 misc_feature 2229..2260 /label=LVA CDS 1650..2228 /label=luxI misc_feature 1650..2267 /label=BBa_C0061 misc_feature 2268..2292 /label=Help:Barcodes CDS 1650..2267 /label=BBa_C0061 misc_feature 1632..2292 /label=BBa_C0261 stem_loop 2312..2355 /label=stem_loop misc_feature 2301..2380 /label=BBa_B0010 terminator 2301..2380 /label=BBa_B0010 stem_loop 2396..2415 /label=T7 TE misc_feature 2422..2422 /label=stop polyA_site 2416..2429 /label=polya misc_feature 2389..2429 /label=BBa_B0012 terminator 2389..2429 /label=BBa_B0012 terminator 2301..2429 /label=BBa_B0015 misc_feature 1632..2429 /label=BBa_K082014 misc_feature 1589..2429 /label=BBa_K176022 promoter 2438..2472 /label=BBa_J23101 misc_feature 2485..2488 /label=conserved RBS 2481..2492 /label=BBa_B0034 misc_feature 2499..2500 /label=prefix misc_feature 2672..2672 /label=T misc_feature 2990..2990 /label=A CDS 2499..3248 /label=luxR misc_feature 2499..3254 /label=BBa_C0062 misc_feature 3255..3279 /label=Help:Barcodes CDS 2499..3254 /label=BBa_C0062 stem_loop 3299..3342 /label=stem_loop misc_feature 3288..3367 /label=BBa_B0010 terminator 3288..3367 /label=BBa_B0010 stem_loop 3383..3402 /label=T7 TE misc_feature 3409..3409 /label=stop polyA_site 3403..3416 /label=polya misc_feature 3376..3416 /label=BBa_B0012 terminator 3376..3416 /label=BBa_B0012 misc_feature 2481..3416 /label=BBa_I0462 misc_feature 2438..3416 /label=BBa_K176025 misc_feature 3425..3477 /label=R0062 promoter 3444..3449 /label=-35 promoter 3466..3471 /label=-10 misc_feature 3478..3496 /label=tetR misc_feature 3477..3477 /label=+1 misc_feature 3425..3444 /label=luxR/HSL promoter 3425..3496 /label=BBa_K176000 misc_feature 2438..3496 /label=BBa_K176028 misc_feature 1589..3496 /label=BBa_K176085 misc_feature 741..3496 /label=BBa_K176237 misc_feature 72..3496 /label=BBa_K176240 ORIGIN 1 gatgaggcta cgtgctttct tactagagtt tatagctagc tcagtcctag gtacaatgct 61 agctactaga gaaagaggag aaatactaga tgactataat gataaaaaaa tcggattttt 121 tggcaattcc atcggaggag tataaaggta ttctaagtct tcgttatcaa gtgtttaagc 181 aaagacttga gtgggactta gttgtagaaa ataaccttga atcagatgag tatgataact 241 caaatgcaga atatatttat gcttgtgatg atactgaaaa tgtaagtgga tgctggcgtt 301 tattacctac aacaggtgat tatatgctga aaagtgtttt tcctgaattg cttggtcaac 361 agagtgctcc caaagatcct aatatagtcg aattaagtcg ttttgctgta ggtaaaaata 421 gctcaaagat aaataactct gctagtgaaa ttacaatgaa actatttgaa gctatatata 481 aacacgctgt tagtcaaggt attacagaat atgtaacagt aacatcaaca gcaatagagc 541 gatttttaaa gcgtattaaa gttccttgtc atcgtattgg agacaaagaa attcatgtat 601 taggtgatac taaatcggtt gtattgtcta tgcctattaa tgaacagttt aaaaaagcag 661 tcttaaatgc tgcaaacgac gaaaactacg ctttagtagc ttaataactc tgatagtgct 721 agtgtagatc tctactagag aaagaggaga aatactagat gtccagatta gataaaagta 781 aagtgattaa cagcgcatta gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc 841 gtaaactcgc ccagaagcta ggtgtagagc agcctacatt gtattggcat gtaaaaaata 901 agcgggcttt gctcgacgcc ttagccattg agatgttaga taggcaccat actcactttt 961 gccctttaga aggggaaagc tggcaagatt ttttacgtaa taacgctaaa agttttagat 1021 gtgctttact aagtcatcgc gatggagcaa aagtacattt aggtacacgg cctacagaaa 1081 aacagtatga aactctcgaa aatcaattag cctttttatg ccaacaaggt ttttcactag 1141 agaatgcatt atatgcactc agcgctgtgg ggcattttac tttaggttgc gtattggaag 1201 atcaagagca tcaagtcgct aaagaagaaa gggaaacacc tactactgat agtatgccgc 1261 cattattacg acaagctatc gaattatttg atcaccaagg tgcagagcca gccttcttat 1321 tcggccttga attgatcata tgcggattag aaaaacaact taaatgtgaa agtgggtccg 1381 ctgcaaacga cgaaaactac gctttagtag cttaataaca ctgatagtgc tagtgtagat 1441 cactactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 1501 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg 1561 tgggcctttc tgcgtttata tactagagtt tatagctagc tcagtcctag gtacaatgct 1621 agctactaga gaaagaggag aaatactaga tgactataat gataaaaaaa tcggattttt 1681 tggcaattcc atcggaggag tataaaggta ttctaagtct tcgttatcaa gtgtttaagc 1741 aaagacttga gtgggactta gttgtagaaa ataaccttga atcagatgag tatgataact 1801 caaatgcaga atatatttat gcttgtgatg atactgaaaa tgtaagtgga tgctggcgtt 1861 tattacctac aacaggtgat tatatgctga aaagtgtttt tcctgaattg cttggtcaac 1921 agagtgctcc caaagatcct aatatagtcg aattaagtcg ttttgctgta ggtaaaaata 1981 gctcaaagat aaataactct gctagtgaaa ttacaatgaa actatttgaa gctatatata 2041 aacacgctgt tagtcaaggt attacagaat atgtaacagt aacatcaaca gcaatagagc 2101 gatttttaaa gcgtattaaa gttccttgtc atcgtattgg agacaaagaa attcatgtat 2161 taggtgatac taaatcggtt gtattgtcta tgcctattaa tgaacagttt aaaaaagcag 2221 tcttaaatgc tgcaaacgac gaaaactacg ctttagtagc ttaataactc tgatagtgct 2281 agtgtagatc tctactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg 2341 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc 2401 accttcgggt gggcctttct gcgtttatat actagagttt acagctagct cagtcctagg 2461 tattatgcta gctactagag aaagaggaga aatactagat gaaaaacata aatgccgacg 2521 acacatacag aataattaat aaaattaaag cttgtagaag caataatgat attaatcaat 2581 gcttatctga tatgactaaa atggtacatt gtgaatatta tttactcgcg atcatttatc 2641 ctcattctat ggttaaatct gatatttcaa tcctagataa ttaccctaaa aaatggaggc 2701 aatattatga tgacgctaat ttaataaaat atgatcctat agtagattat tctaactcca 2761 atcattcacc aattaattgg aatatatttg aaaacaatgc tgtaaataaa aaatctccaa 2821 atgtaattaa agaagcgaaa acatcaggtc ttatcactgg gtttagtttc cctattcata 2881 cggctaacaa tggcttcgga atgcttagtt ttgcacattc agaaaaagac aactatatag 2941 atagtttatt tttacatgcg tgtatgaaca taccattaat tgttccttct ctagttgata 3001 attatcgaaa aataaatata gcaaataata aatcaaacaa cgatttaacc aaaagagaaa 3061 aagaatgttt agcgtgggca tgcgaaggaa aaagctcttg ggatatttca aaaatattag 3121 gttgcagtga gcgtactgtc actttccatt taaccaatgc gcaaatgaaa ctcaatacaa 3181 caaaccgctg ccaaagtatt tctaaagcaa ttttaacagg agcaattgat tgcccatact 3241 ttaaaaatta ataacactga tagtgctagt gtagatcact actagagcca ggcatcaaat 3301 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa 3361 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact 3421 agagacctgt aggatcgtac aggtttacgc aagaaaatgg tttgttatag tcgaatatcc 3481 ctatcagtga tagaga //