Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Tyler Lee
Date created: 2015-09-10 11:00:00
Date modified: 2016-10-02 06:33:24
PCquad Mutant 10
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1763421_sequence (Version 1) |
Description
Subunit of a 12-subunit protein cage with a thrombin cleavage sequence inserted between residues 243 and 248 (ALPSAE to ALPGLVPRGSGSAE).Notes
Considered the area to insert the cleavage sequence. This mutation was selected by examining the secondary structure to ensure minimal disruption of existing alpha-helices or beta sheets, sites were sandwiched by glycine residues, and used PyMOL to check if the site would be accessible to the protease. Geometric accessibility by the protease thrombin and minimal speculated extent of conformational changes by insertion.Source
Bromoperoxidase (trimer) and M1 Virus Matrix Protein (dimer) linked with an alpha helix.| Sequence Annotation | Location | Component / Role(s) |
| T7 Promoter RBS Scar Double Stop Start 6x His Tag PCquad Mutant 10 | 1,22 24,28 34,39 1425,1430 40,42 1386,1400 40,1385 | promoter feature/promoter feature/rbs ribosome_entry_site feature/scar feature/stop stop_codon feature/start start_codon sequence_feature feature/misc engineered_region feature/BioBrick |