LOCUS       BBa_K1763421            1430 bp    DNA     linear       03-May-2024
DEFINITION  PCquad Mutant 10
ACCESSION   BBa_K1763421
VERSION     BBa_K1763421.1  
FEATURES             Location/Qualifiers
     promoter        1..22
                     /label=T7 Promoter
     RBS             24..28
                     /label=RBS
     misc_feature    34..39
                     /label=Scar
     misc_feature    1425..1430
                     /label=Double Stop
     misc_feature    40..42
                     /label=Start
     misc_feature    1386..1400
                     /label=6x His Tag
     misc_feature    40..1385
                     /label=PCquad Mutant 10
ORIGIN
        1 taatacgact cactataggg aaagaggaga aatactagat gccgttcatt accgtaggac
       61 aagaaaattc tacgtctatt gatttgtatt atgaggacca tggtaccggt acgccggtgg
      121 ttctcatcca cggctttccg ctatcaggac attcttggga gcgtcagagc gctgcgcttt
      181 tagatgccgg tgctcgtgta ataacgtacg atagacgcgg ttttggccag agctctcagc
      241 caacgactgg atacgattat gacaccttcg ccgccgattt aaatactgtt ctggaaaccc
      301 tggatcttca ggatgcggtc ttagttggtt ttagtatggg cacaggtgaa gttgcccgct
      361 acgtcagttc ttatggcact gctcgtattg cagcggtagc ttttctggct agcttagaac
      421 cctttctatt aaaaaccgat gataatccgg atggggcggc tccacaggag tttttcgacg
      481 gaattgtggc cgctgtgaaa gccgatagat atgctttcta tactgggttt ttcaatgact
      541 tctataattt agatgaaaac ctgggaacac gcatctcgga agaggctgta cggaactcat
      601 ggaatactgc ggcatctggc ggattctttg ctgccgcagc cgcgccgacc acttggtata
      661 cagattttcg tgcggacatt cctagaattg atgtacctgc cctgattctg cacggtacgg
      721 gcgaccgtac cttaccgatt gagaacactg cccgcgtctt tcataaagct cttccgggcc
      781 tggttccccg cggttcaggc tccgctgagt acgtagaggt tgaaggggca cctcatggtc
      841 tgttatggac tcatgctgaa gaagtcaaca cagccctgct tgctttcctt gcgaaggctc
      901 aagaagccca aaagcagaaa ttactgaccg aagtggaaac ttatgtacta tccatcatac
      961 cgtctggtcc ccttaaagca gaaatcgcac aacgtctgga ggatgtcttc gcgggcaaaa
     1021 atacagacct ggaggtgctg atggaatggc ttaagacccg accgattctg tcaccgttaa
     1081 cgaagggtat cctaggattc gtttttaccc tgacggtgcc cagtgagcgc ggactgcaac
     1141 gaagaagatt cgtccaaaac gcattaaacg ggaatggtga cccaaacaat atggacaaag
     1201 cggtgaagct gtatcgaaaa cttaagcgcg aaataacatt ccatggggcc aaagaaatta
     1261 gcctgagcta ttccgctggg gcgcttgctt cttgtatggg tttaatatat aaccgaatgg
     1321 gtgcggtcac cacggaagtc gcgttcggat tagtatgcgc gacatgcgag cagattgcag
     1381 actctcagca tagaagccat cgtcagcatc accaccatca ccattaataa
//