BBa_K1763421 1 BBa_K1763421 PCquad Mutant 10 2015-09-10T11:00:00Z 2016-10-02T06:33:24Z Bromoperoxidase (trimer) and M1 Virus Matrix Protein (dimer) linked with an alpha helix. Subunit of a 12-subunit protein cage with a thrombin cleavage sequence inserted between residues 243 and 248 (ALPSAE to ALPGLVPRGSGSAE). false false _2185_ 27134 27528 9 false Considered the area to insert the cleavage sequence. This mutation was selected by examining the secondary structure to ensure minimal disruption of existing alpha-helices or beta sheets, sites were sandwiched by glycine residues, and used PyMOL to check if the site would be accessible to the protease. Geometric accessibility by the protease thrombin and minimal speculated extent of conformational changes by insertion. false Tyler Lee annotation2467609 1 RBS range2467609 1 24 28 annotation2467612 1 Start range2467612 1 40 42 annotation2467628 1 6x His Tag range2467628 1 1386 1400 annotation2467630 1 PCquad Mutant 10 range2467630 1 40 1385 annotation2467608 1 T7 Promoter range2467608 1 1 22 annotation2467611 1 Double Stop range2467611 1 1425 1430 annotation2467610 1 Scar range2467610 1 34 39 BBa_K1763421_sequence 1 taatacgactcactatagggaaagaggagaaatactagatgccgttcattaccgtaggacaagaaaattctacgtctattgatttgtattatgaggaccatggtaccggtacgccggtggttctcatccacggctttccgctatcaggacattcttgggagcgtcagagcgctgcgcttttagatgccggtgctcgtgtaataacgtacgatagacgcggttttggccagagctctcagccaacgactggatacgattatgacaccttcgccgccgatttaaatactgttctggaaaccctggatcttcaggatgcggtcttagttggttttagtatgggcacaggtgaagttgcccgctacgtcagttcttatggcactgctcgtattgcagcggtagcttttctggctagcttagaaccctttctattaaaaaccgatgataatccggatggggcggctccacaggagtttttcgacggaattgtggccgctgtgaaagccgatagatatgctttctatactgggtttttcaatgacttctataatttagatgaaaacctgggaacacgcatctcggaagaggctgtacggaactcatggaatactgcggcatctggcggattctttgctgccgcagccgcgccgaccacttggtatacagattttcgtgcggacattcctagaattgatgtacctgccctgattctgcacggtacgggcgaccgtaccttaccgattgagaacactgcccgcgtctttcataaagctcttccgggcctggttccccgcggttcaggctccgctgagtacgtagaggttgaaggggcacctcatggtctgttatggactcatgctgaagaagtcaacacagccctgcttgctttccttgcgaaggctcaagaagcccaaaagcagaaattactgaccgaagtggaaacttatgtactatccatcataccgtctggtccccttaaagcagaaatcgcacaacgtctggaggatgtcttcgcgggcaaaaatacagacctggaggtgctgatggaatggcttaagacccgaccgattctgtcaccgttaacgaagggtatcctaggattcgtttttaccctgacggtgcccagtgagcgcggactgcaacgaagaagattcgtccaaaacgcattaaacgggaatggtgacccaaacaatatggacaaagcggtgaagctgtatcgaaaacttaagcgcgaaataacattccatggggccaaagaaattagcctgagctattccgctggggcgcttgcttcttgtatgggtttaatatataaccgaatgggtgcggtcaccacggaagtcgcgttcggattagtatgcgcgacatgcgagcagattgcagactctcagcatagaagccatcgtcagcatcaccaccatcaccattaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z