LOCUS       BBa_K1767002            2666 bp    DNA     linear       21-May-2024
DEFINITION  P(Lac)IQ RBS Aiia RBS LuxR ter ter luxpR RBS tetR ter ter
ACCESSION   BBa_K1767002
VERSION     BBa_K1767002.1  
FEATURES             Location/Qualifiers
     promoter        1..37
                     /label=BBa_I14032/p(Lac) IQ
     misc_feature    3..8
                     /label=-35
     misc_feature    26..31
                     /label=-10
     RBS             44..50
                     /label=RBS/BBa_B0034
     CDS             59..812
                     /label=AiiA
     RBS             820..832
                     /label=RBS
     CDS             838..1590
                     /label=LuxR
     stem_loop       1627..1707
                     /label=BBa_B0010/term
     stem_loop       1715..1756
                     /label=BBa_B0012/term
     promoter        1764..1818
                     /label=BBa_R0062/pLux promoter
     misc_feature    1..1818
                     /label=BBa_K1767001
     misc_feature    1831..1834
                     /label=conserved
     RBS             1827..1838
                     /label=BBa_B0034
     CDS             1848..2464
                     /label=tetR
     misc_feature    2465..2498
                     /label=SsrA
     misc_feature    2505..2529
                     /label=Help:Barcodes
     CDS             1845..2504
                     /label=BBa_C0040
     stem_loop       2549..2592
                     /label=stem_loop
     misc_feature    2538..2617
                     /label=BBa_B0010
     terminator      2538..2617
                     /label=BBa_B0010
     stem_loop       2633..2652
                     /label=T7 TE
     misc_feature    2659..2659
                     /label=stop
     polyA_site      2653..2666
                     /label=polya
     misc_feature    2626..2666
                     /label=BBa_B0012
     terminator      2626..2666
                     /label=BBa_B0012
     misc_feature    1827..2666
                     /label=BBa_P0440
ORIGIN
        1 tggtgcaaaa cctttcgcgg tatggcatga tagcgcctac aaagagggga catactagat
       61 gacagtaaag aagctttatt tcgtcccagc aggtcgttgt atgttggatc attcgtctgt
      121 taatagtaca ttaacaccag gagaattatt agacttaccg gtttggtgtt atcttttgga
      181 gactgaagaa ggacctattt tagtagatac aggtatgcca gaaagtgcag ttaataatga
      241 aggtcttttt aacggtacat ttgtcgaagg gcaggtttta ccgaaaatga ctgaagaaga
      301 tagaatcgtg aatattttaa aacgggttgg ttatgagccg gaagaccttc tttatattat
      361 tagttctcac ttgcattttg atcatgcagg aggaaatggc gcttttataa atacaccaat
      421 cattgtacag cgtgctgaat atgaggcggc gcagcatagc gaagaatatt tgaaagaatg
      481 tatattgccg aatttaaact acaaaatcat tgaaggtgat tatgaagtcg taccaggagt
      541 tcaattattg catacaccag gccatactcc agggcatcaa tcgctattaa ttgagacaga
      601 aaaatccggt cctgtattat taacgattga tgcatcgtat acgaaagaga attttgaaaa
      661 tgaagtgcca tttgcgggat ttgattcaga attagcttta tcttcaatta aacgtttaaa
      721 agaagtggtg atgaaagaga agccgattgt tttctttgga catgatatag agcaggaaag
      781 gggatgtaaa gtgttccctg aatatatata ataatagaga aagaggagaa atactagatg
      841 aaaaacataa atgccgacga cacatacaga ataattaata aaattaaagc ttgtagaagc
      901 aataatgata ttaatcaatg cttatctgat atgactaaaa tggtacattg tgaatattat
      961 ttactcgcga tcatttatcc tcattctatg gttaaatctg atatttcaat cctagataat
     1021 taccctaaaa aatggaggca atattatgat gacgctaatt taataaaata tgatcctata
     1081 gtagattatt ctaactccaa tcattcacca attaattgga atatatttga aaacaatgct
     1141 gtaaataaaa aatctccaaa tgtaattaaa gaagcgaaaa catcaggtct tatcactggg
     1201 tttagtttcc ctattcatac ggctaacaat ggcttcggaa tgcttagttt tgcacattca
     1261 gaaaaagaca actatataga tagtttattt ttacatgcgt gtatgaacat accattaatt
     1321 gttccttctc tagttgataa ttatcgaaaa ataaatatag caaataataa atcaaacaac
     1381 gatttaacca aaagagaaaa agaatgttta gcgtgggcat gcgaaggaaa aagctcttgg
     1441 gatatttcaa aaatattagg ttgcagtgag cgtactgtca ctttccattt aaccaatgcg
     1501 caaatgaaac tcaatacaac aaaccgctgc caaagtattt ctaaagcaat tttaacagga
     1561 gcaattgatt gcccatactt taaaaattaa taacactgat agtgctagtg tagatcacta
     1621 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     1681 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     1741 ctttctgcgt ttatatacta gagacctgta ggatcgtaca ggtttacgca agaaaatggt
     1801 ttgttatagt cgaataaata ctagagaaag aggagaaata ctagatgtcc agattagata
     1861 aaagtaaagt gattaacagc gcattagagc tgcttaatga ggtcggaatc gaaggtttaa
     1921 caacccgtaa actcgcccag aagctaggtg tagagcagcc tacattgtat tggcatgtaa
     1981 aaaataagcg ggctttgctc gacgccttag ccattgagat gttagatagg caccatactc
     2041 acttttgccc tttagaaggg gaaagctggc aagatttttt acgtaataac gctaaaagtt
     2101 ttagatgtgc tttactaagt catcgcgatg gagcaaaagt acatttaggt acacggccta
     2161 cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt tttatgccaa caaggttttt
     2221 cactagagaa tgcattatat gcactcagcg ctgtggggca ttttacttta ggttgcgtat
     2281 tggaagatca agagcatcaa gtcgctaaag aagaaaggga aacacctact actgatagta
     2341 tgccgccatt attacgacaa gctatcgaat tatttgatca ccaaggtgca gagccagcct
     2401 tcttattcgg ccttgaattg atcatatgcg gattagaaaa acaacttaaa tgtgaaagtg
     2461 ggtccgctgc aaacgacgaa aactacgctt tagtagctta ataacactga tagtgctagt
     2521 gtagatcact actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     2581 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
     2641 ttcgggtggg cctttctgcg tttata
//