BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation1690
1
polya
range1690
1
28
41
annotation1687
1
stop
range1687
1
34
34
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_R0062
1
lux pR
Promoter (luxR & HSL regulated -- lux pR)
2003-01-31T12:00:00Z
2015-05-08T01:14:15Z
<em>V. fischeri</em>
Released HQ 2013
Promoter activated by LuxR in concert with HSL</p> <p>The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
false
true
_1_
0
24
7
In stock
false
<P> <P>This promoter is based on the <em>Vibrio fischeri </em>quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <p><img src="<bb_file>Image1.gif</bb_file>" width="614" height="362"><P>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
annotation2047
1
-10
range2047
1
42
47
annotation7070
1
BBa_R0062
range7070
1
1
55
annotation2048
1
start
range2048
1
53
53
annotation2046
1
-35
range2046
1
20
25
annotation2045
1
LuxR/HSL
range2045
1
1
20
BBa_K1768002
1
BBa_K1768002
Quorum sensing-inducible mxiE transcription factor
2015-09-12T11:00:00Z
2015-09-13T01:13:35Z
Moon, Tae Seok, et al. "Genetic programs constructed from layered logic gates in single cells." Nature 491.7423 (2012): 249-253.
The LuxR receptor binds to the lux pR promoter to induce expression of the mxiE transcription factor (BBa_K1325001). mxiE forms a protein-protein interaction with the chaperone protein ipgC (BBa_K1325000) in order to transactivate the pipaH promoter (BBa_K1325004) via an "AND" operation.
false
false
_2190_
24907
24907
9
false
None
false
Steven Hussey
component2453283
1
BBa_K1325001
component2453286
1
BBa_B0012
component2453276
1
BBa_R0062
component2453284
1
BBa_B0010
component2453282
1
BBa_B0034
annotation2453286
1
BBa_B0012
range2453286
1
934
974
annotation2453282
1
BBa_B0034
range2453282
1
64
75
annotation2453276
1
BBa_R0062
range2453276
1
1
55
annotation2453284
1
BBa_B0010
range2453284
1
846
925
annotation2453283
1
BBa_K1325001
range2453283
1
82
837
BBa_K1325001
1
BBa_K1325001
ranscriptionfactor
2014-09-26T11:00:00Z
2015-05-08T01:09:54Z
gene clusters encoding type III secretion systems
ranscriptionfactor，the ipgC???mxiE complex activates transcriptionfrom the pipaH* promoter
false
false
_1700_
0
23253
9
In stock
false
no
false
Ke Xu
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_R0062_sequence
1
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K1325001_sequence
1
atgagtaaatataaaggcctgaacaccagcaacatgttctacatctacagctctggtcatgaaccggtgaacgttgaactggtgaaagataaagaacgtaacatcatcgaactggcaccggcgtggaaaggctttttctttgtgcgtaaccagaacatcaaattcagcgataacgttaactaccactaccgcttcaacatcaactcttgcgcaaaattcctggcgttttgggattattttagcggcgccctggttgaacattctcacgcagaaaaatgcatccatttctaccacgaaaacgatctgcgtgatagctgtaatacggaatctatgctggataaactgatgctgcgcttcatttttagtagcgatcagaacgtgtctaatgccctggcaatgatccgtatgaccgaaagttatcatctggttctgtacctgctgcgtacgattgaaaaagaaaaagaagtgcgcatcaaaagcctgaccgaacactatggcgtttctgaagcgtactttcgtagtctgtgtcgcaaagcgctgggtgccaaagtgaaagaacagctgaacacgtggcgcctggtgaatggcctgctggatgttttcctgcataaccagaccattacgagcgcggccatgaacaatggttatgcgtctaccagtcacttcagcaatgaaattaaaacgcgtctgggctttagtgcccgcgaactgagcaacatcaccttcctggtgaagaaaattaatgaaaaaatctaa
BBa_K1768002_sequence
1
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatgagtaaatataaaggcctgaacaccagcaacatgttctacatctacagctctggtcatgaaccggtgaacgttgaactggtgaaagataaagaacgtaacatcatcgaactggcaccggcgtggaaaggctttttctttgtgcgtaaccagaacatcaaattcagcgataacgttaactaccactaccgcttcaacatcaactcttgcgcaaaattcctggcgttttgggattattttagcggcgccctggttgaacattctcacgcagaaaaatgcatccatttctaccacgaaaacgatctgcgtgatagctgtaatacggaatctatgctggataaactgatgctgcgcttcatttttagtagcgatcagaacgtgtctaatgccctggcaatgatccgtatgaccgaaagttatcatctggttctgtacctgctgcgtacgattgaaaaagaaaaagaagtgcgcatcaaaagcctgaccgaacactatggcgtttctgaagcgtactttcgtagtctgtgtcgcaaagcgctgggtgccaaagtgaaagaacagctgaacacgtggcgcctggtgaatggcctgctggatgttttcctgcataaccagaccattacgagcgcggccatgaacaatggttatgcgtctaccagtcacttcagcaatgaaattaaaacgcgtctgggctttagtgcccgcgaactgagcaacatcaccttcctggtgaagaaaattaatgaaaaaatctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z