LOCUS       BBa_K177030             1394 bp    DNA     linear       01-Jun-2024
DEFINITION  MgtC promoter testing device without pho
ACCESSION   BBa_K177030
VERSION     BBa_K177030.1  
FEATURES             Location/Qualifiers
     misc_feature    339..357
                     /label=Non-canonical PhoP binding site
     promoter        1..510
                     /label=BBa_K177021
     RBS             519..531
                     /label=RBS-3\Weak
     RBS             525..528
                     /label=RBS
     misc_feature    519..531
                     /label=BBa_B0032
     RBS             519..531
                     /label=BBa_B0032
     CDS             538..1257
                     /label=GFP protein
     CDS             538..1257
                     /label=BBa_E0040
     stem_loop       1277..1320
                     /label=stem_loop
     misc_feature    1266..1345
                     /label=BBa_B0010
     terminator      1266..1345
                     /label=BBa_B0010
     stem_loop       1361..1380
                     /label=T7 TE
     misc_feature    1387..1387
                     /label=stop
     polyA_site      1381..1394
                     /label=polya
     misc_feature    1354..1394
                     /label=BBa_B0012
     terminator      1354..1394
                     /label=BBa_B0012
     misc_feature    519..1394
                     /label=BBa_E0240
ORIGIN
        1 agtccatttt ttctggaaga ataagtacgt gctatattta gcacacgaca aatttctgcc
       61 ctgaggcaat tagcgtcgtg ttaacggcgg aggaaataaa gcgtgtttaa acatgaacat
      121 gacgattacc tcgcgacaat gcgcgtgttg taacattatt gggatgacaa tcgtcacaag
      181 gggagattgc tgcccaccgg ttcggcaagc agcgcaggaa tagcgtgcgt taacttgcca
      241 gatgatttac gtaaaagtga ctgtccaaca tattactcct gcatgaattt gcgcttatta
      301 tagtcagcca atatgttccg taaagtgcaa aggcgggttt agtgacgttc tgtttaagtt
      361 tgtttgatat tttttcttat gacaaaaaaa tgacaatatt atgtcgataa caagctcgtc
      421 tgaaatcatt aaacaataaa ttcctgctta ataccggcga tagctctgtt atgatgcctg
      481 cacgtaaaaa aataaaacaa catgacgagg tactagagtc acacaggaaa gtactagatg
      541 cgtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat
      601 gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa
      661 cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc
      721 actactttcg gttatggtgt tcaatgcttt gcgagatacc cagatcatat gaaacagcat
      781 gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa
      841 gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat
      901 agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg
      961 gaatacaact ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc
     1021 aaagttaact tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat
     1081 tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg
     1141 tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt
     1201 gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa ataataatac
     1261 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
     1321 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc
     1381 tttctgcgtt tata
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