LOCUS       BBa_K177034             1827 bp    DNA     linear       03-May-2024
DEFINITION  Operon 1 part 1 + GFP
ACCESSION   BBa_K177034
VERSION     BBa_K177034.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..221
                     /label=RBS-3\Weak
     RBS             215..218
                     /label=RBS
     misc_feature    209..221
                     /label=BBa_B0032
     RBS             209..221
                     /label=BBa_B0032
     CDS             228..941
                     /label=cIts
     CDS             228..941
                     /label=BBa_K177050
     RBS             950..964
                     /label=RBS-1\Strong
     RBS             957..961
                     /label=RBS
     misc_feature    950..964
                     /label=BBa_B0030
     RBS             950..964
                     /label=BBa_B0030
     CDS             971..1690
                     /label=GFP protein
     CDS             971..1690
                     /label=BBa_E0040
     stem_loop       1710..1753
                     /label=stem_loop
     misc_feature    1699..1778
                     /label=BBa_B0010
     terminator      1699..1778
                     /label=BBa_B0010
     stem_loop       1794..1813
                     /label=T7 TE
     misc_feature    1820..1820
                     /label=stop
     polyA_site      1814..1827
                     /label=polya
     misc_feature    1787..1827
                     /label=BBa_B0012
     terminator      1787..1827
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagtc acacaggaaa gtactagatg agcacaaaaa
      241 agaaaccatt aacacaagag cagcttgagg acgcacgtcg ccttaaagca atttatgaaa
      301 aaaagaaaaa tgaacttggc ttatcccagg aatctgtcgc agacaagatg gggatggggc
      361 agtcaggcgt tggtgcttta tttaatggca tcaatgcatt aaatgcttat aacgccgcat
      421 tgcttacaaa aattctcaaa gttagcgttg aagaatttag cccttcaatc gccagagaaa
      481 tctacgagat gtatgaagcg gttagtatgc agccgtcact tagaagtgag tatgagtacc
      541 ctgttttttc tcatgttcag gcagggatgt tctcacctga gcttagaacc tttaccaaag
      601 gtgatgcgga gagatgggta agcacaacca aaaaagccag tgattctgca ttctggcttg
      661 aggttgaagg taattccatg accgcaccaa caggctccaa gccaagcttt cctgacggaa
      721 tgttaattct cgttgaccct gagcaggctg ttgagccagg tgatttctgc atagccagac
      781 ttgggggtga tgagtttacc ttcaagaaac tgatcaggga tagcggtcag gtgtttttac
      841 aaccactaaa cccacagtac ccaatgatcc catgcaatga gagttgttcc gttgtgggga
      901 aagttatcgc tagtcagtgg cctgaagaga cgtttggctg atactagaga ttaaagagga
      961 gaaatactag atgcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga
     1021 attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc
     1081 aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg
     1141 gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca
     1201 tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac
     1261 tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga
     1321 tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct
     1381 tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca
     1441 aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca
     1501 actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga
     1561 caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca
     1621 catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata
     1681 caaataataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
     1741 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac
     1801 cttcgggtgg gcctttctgc gtttata
//