LOCUS BBa_K177038 3933 bp DNA linear 07-May-2024 DEFINITION Bistable switch 1 testing device ACCESSION BBa_K177038 VERSION BBa_K177038.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 RBS 209..221 /label=RBS-3\Weak RBS 215..218 /label=RBS misc_feature 209..221 /label=BBa_B0032 RBS 209..221 /label=BBa_B0032 CDS 228..941 /label=cIts CDS 228..941 /label=BBa_K177050 RBS 950..964 /label=RBS-1\Strong RBS 957..961 /label=RBS misc_feature 950..964 /label=BBa_B0030 RBS 950..964 /label=BBa_B0030 CDS 971..1690 /label=GFP protein CDS 971..1690 /label=BBa_E0040 stem_loop 1710..1753 /label=stem_loop misc_feature 1699..1778 /label=BBa_B0010 terminator 1699..1778 /label=BBa_B0010 stem_loop 1794..1813 /label=T7 TE misc_feature 1820..1820 /label=stop polyA_site 1814..1827 /label=polya misc_feature 1787..1827 /label=BBa_B0012 terminator 1787..1827 /label=BBa_B0012 misc_feature 1..1827 /label=BBa_K177034 promoter 1873..1878 /label=-10 promoter 1850..1855 /label=-35 misc_feature 1860..1876 /label=OR1 misc_feature 1836..1852 /label=OR2 misc_feature 1836..1884 /label=BBa_R0051 promoter 1836..1884 /label=BBa_R0051 RBS 1893..1905 /label=RBS-3\Weak RBS 1899..1902 /label=RBS misc_feature 1893..1905 /label=BBa_B0032 RBS 1893..1905 /label=BBa_B0032 misc_feature 3001..3039 /label=LVA CDS 1912..3039 /label=lacI-LVA misc_feature 1912..3039 /label=BBa_C0012 misc_feature 3040..3064 /label=Help:Barcodes CDS 1912..3039 /label=BBa_C0012 misc_feature 1836..3064 /label=BBa_K177012 misc_feature 3077..3080 /label=conserved RBS 3073..3084 /label=BBa_B0034 CDS 3091..3765 /label=mrfp1 misc_feature 3772..3796 /label=Help:Barcodes CDS 3091..3796 /label=BBa_E1010 stem_loop 3816..3859 /label=stem_loop misc_feature 3805..3884 /label=BBa_B0010 terminator 3805..3884 /label=BBa_B0010 stem_loop 3900..3919 /label=T7 TE misc_feature 3926..3926 /label=stop polyA_site 3920..3933 /label=polya misc_feature 3893..3933 /label=BBa_B0012 terminator 3893..3933 /label=BBa_B0012 misc_feature 3073..3933 /label=BBa_K094130 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagtc acacaggaaa gtactagatg agcacaaaaa 241 agaaaccatt aacacaagag cagcttgagg acgcacgtcg ccttaaagca atttatgaaa 301 aaaagaaaaa tgaacttggc ttatcccagg aatctgtcgc agacaagatg gggatggggc 361 agtcaggcgt tggtgcttta tttaatggca tcaatgcatt aaatgcttat aacgccgcat 421 tgcttacaaa aattctcaaa gttagcgttg aagaatttag cccttcaatc gccagagaaa 481 tctacgagat gtatgaagcg gttagtatgc agccgtcact tagaagtgag tatgagtacc 541 ctgttttttc tcatgttcag gcagggatgt tctcacctga gcttagaacc tttaccaaag 601 gtgatgcgga gagatgggta agcacaacca aaaaagccag tgattctgca ttctggcttg 661 aggttgaagg taattccatg accgcaccaa caggctccaa gccaagcttt cctgacggaa 721 tgttaattct cgttgaccct gagcaggctg ttgagccagg tgatttctgc atagccagac 781 ttgggggtga tgagtttacc ttcaagaaac tgatcaggga tagcggtcag gtgtttttac 841 aaccactaaa cccacagtac ccaatgatcc catgcaatga gagttgttcc gttgtgggga 901 aagttatcgc tagtcagtgg cctgaagaga cgtttggctg atactagaga ttaaagagga 961 gaaatactag atgcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga 1021 attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc 1081 aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg 1141 gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca 1201 tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac 1261 tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga 1321 tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct 1381 tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca 1441 aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca 1501 actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga 1561 caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca 1621 catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata 1681 caaataataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg 1741 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac 1801 cttcgggtgg gcctttctgc gtttatatac tagagtaaca ccgtgcgtgt tgactatttt 1861 acctctggcg gtgataatgg ttgctactag agtcacacag gaaagtacta gatggtgaat 1921 gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt 1981 tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg 2041 gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag 2101 tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc 2161 gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa 2221 cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt 2281 gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc 2341 actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt 2401 ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag 2461 caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc 2521 tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg 2581 agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact 2641 gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc 2701 gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca 2761 tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc 2821 gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc 2881 gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc 2941 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag 3001 gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg ctagtgtaga 3061 tctctactag agaaagagga gaaatactag atggcttcct ccgaagacgt tatcaaagag 3121 ttcatgcgtt tcaaagttcg tatggaaggt tccgttaacg gtcacgagtt cgaaatcgaa 3181 ggtgaaggtg aaggtcgtcc gtacgaaggt acccagaccg ctaaactgaa agttaccaaa 3241 ggtggtccgc tgccgttcgc ttgggacatc ctgtccccgc agttccagta cggttccaaa 3301 gcttacgtta aacacccggc tgacatcccg gactacctga aactgtcctt cccggaaggt 3361 ttcaaatggg aacgtgttat gaacttcgaa gacggtggtg ttgttaccgt tacccaggac 3421 tcctccctgc aagacggtga gttcatctac aaagttaaac tgcgtggtac caacttcccg 3481 tccgacggtc cggttatgca gaaaaaaacc atgggttggg aagcttccac cgaacgtatg 3541 tacccggaag acggtgctct gaaaggtgaa atcaaaatgc gtctgaaact gaaagacggt 3601 ggtcactacg acgctgaagt taaaaccacc tacatggcta aaaaaccggt tcagctgccg 3661 ggtgcttaca aaaccgacat caaactggac atcacctccc acaacgaaga ctacaccatc 3721 gttgaacagt acgaacgtgc tgaaggtcgt cactccaccg gtgcttaata acgctgatag 3781 tgctagtgta gatcgctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag 3841 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg 3901 gctcaccttc gggtgggcct ttctgcgttt ata //