LOCUS       BBa_K1773006            1140 bp    DNA     linear       21-May-2024
DEFINITION  pLac with RBS attached to cI gene.
ACCESSION   BBa_K1773006
VERSION     BBa_K1773006.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    215..218
                     /label=conserved
     RBS             209..222
                     /label=BBa_B0031
     CDS             232..939
                     /label=cI lambda
     misc_feature    940..972
                     /label=LVA
     misc_feature    979..1003
                     /label=Help:Barcodes
     CDS             229..978
                     /label=BBa_C0051
     stem_loop       1023..1066
                     /label=stem_loop
     misc_feature    1012..1091
                     /label=BBa_B0010
     terminator      1012..1091
                     /label=BBa_B0010
     stem_loop       1107..1126
                     /label=T7 TE
     misc_feature    1133..1133
                     /label=stop
     polyA_site      1127..1140
                     /label=polya
     misc_feature    1100..1140
                     /label=BBa_B0012
     terminator      1100..1140
                     /label=BBa_B0012
     misc_feature    209..1140
                     /label=BBa_P0151
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagtc acacaggaaa cctactagat gagcacaaaa
      241 aagaaaccat taacacaaga gcagcttgag gacgcacgtc gccttaaagc aatttatgaa
      301 aaaaagaaaa atgaacttgg cttatcccag gaatctgtcg cagacaagat ggggatgggg
      361 cagtcaggcg ttggtgcttt atttaatggc atcaatgcat taaatgctta taacgccgca
      421 ttgcttgcaa aaattctcaa agttagcgtt gaagaattta gcccttcaat cgccagagaa
      481 atctacgaga tgtatgaagc ggttagtatg cagccgtcac ttagaagtga gtatgagtac
      541 cctgtttttt ctcatgttca ggcagggatg ttctcacctg agcttagaac ctttaccaaa
      601 ggtgatgcgg agagatgggt aagcacaacc aaaaaagcca gtgattctgc attctggctt
      661 gaggttgaag gtaattccat gaccgcacca acaggctcca agccaagctt tcctgacgga
      721 atgttaattc tcgttgaccc tgagcaggct gttgagccag gtgatttctg catagccaga
      781 cttgggggtg atgagtttac cttcaagaaa ctgatcaggg atagcggtca ggtgttttta
      841 caaccactaa acccacagta cccaatgatc ccatgcaatg agagttgttc cgttgtgggg
      901 aaagttatcg ctagtcagtg gcctgaagag acgtttggcg ctgcaaacga cgaaaactac
      961 gctttagtag cttaataacg ctgatagtgc tagtgtagat cgctactaga gccaggcatc
     1021 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     1081 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//