Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sarunas Tumas
Date created: 2015-08-25 11:00:00
Date modified: 2015-09-12 07:15:36
Cas3 gene with pLux/cI right promoter and strong RBS
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K1773019_sequence (Version 1) |
Description
Gene encoding I-F type CRISPR_Cas system Cas3 protein, which is an helicase and ssDNA nuclease. Attached there is a pLux/cI right promoter and strong RBS site. In the I-F type CRISPR-Cas system Cascade complex distinguishes target DNA, and rectruits Cas3 protein, which acts as a nuclease and helicase, which hidrolises ssDNA in a 3'-5' direction.Notes
No.Source
I-F type CRISPR-Cas system was cloned from Aggregatibacter actinomycetemcomitans D7-S strand.| Access | Instance | Definition |
| public public | BBa_K1773016 BBa_K1773003 | BBa_K1773016 BBa_K1773003 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1773016 BBa_K1773003 | 1,93 100,3385 | BBa_K1773016 BBa_K1773003 |