BBa_K1773020

BBa_K1773020 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1773020
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sarunas Tumas
Date created: 2015-08-25 11:00:00
Date modified: 2015-08-26 03:52:20

Cas3 gene with pLux/cI right promoter and Medium RBS



Types
DnaRegion

Roles
engineered_region

Generator

Sequences BBa_K1773020_sequence (Version 1)

Description

Gene encoding I-F type CRISPR_Cas system Cas3 protein, which is an helicase and ssDNA nuclease. Attached there is a pLux/cI right promoter and Medium RBS site. In the I-F type CRISPR-Cas system Cascade complex distinguishes target DNA, and rectruits Cas3 protein, which acts as a nuclease and helicase, which hidrolises ssDNA in a 3'-5' direction.

Notes

no

Source

I-F type CRISPR-Cas system was cloned from Aggregatibacter actinomycetemcomitans D7-S strand.

igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1773020/1