LOCUS       BBa_K1773020            3383 bp    DNA     linear       06-May-2024
DEFINITION  Cas3 gene with pLux/cI right promoter and Medium RBS
ACCESSION   BBa_K1773020
VERSION     BBa_K1773020.1  
FEATURES             Location/Qualifiers
     promoter        1..78
                     /label=BBa_K1773015
     RBS             79..91
                     /label=RBS-3\Weak
     RBS             85..88
                     /label=RBS
     misc_feature    79..91
                     /label=BBa_B0032
     RBS             79..91
                     /label=BBa_B0032
     promoter        1..91
                     /label=BBa_K1773017
     CDS             98..3383
                     /label=BBa_K1773003
ORIGIN
        1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga atacctctgg
       61 cggtgataca catgcatatc acacaggaaa gtactagatg aatatcttac ttgttagcca
      121 atgccaaaaa aatgcgttaa cggaaacccg ccgcattctc gatcaatttg ccgaacggtg
      181 cggtgatcgc gtatggcaaa cacccattac acaagctggg ttagacaccc tgcataaaat
      241 gttacgacaa acggcgcgta aaaatacagc agtcgcttgt tattggacac atgggaaaaa
      301 tttaacggaa ttgctttgga ttgtggggga taaatcacaa tttaacgaag aagggcgtgt
      361 gccgacaaac cgaaccaagc gcaatatttt gcgcgccgaa gatgaaggcg gttggttaca
      421 agcctattcg atccaaattg tgaccgcact tgccgcgtta ttacacgatt taggcaaggc
      481 gaacaaagcc tttcagttaa aattgaaacc caatgcgcct tttcacgcgg attattttcg
      541 tcacgagtgg atttcagccc gattgtttga agccatgatt gctggttgta aaaccgatca
      601 ggagtggctg tcacgtttgg ctaattgggc tgaatataaa gaaaattggt tagcttcgct
      661 taatattgat gataatgaag ctgattttag tcatttgccg cctttggcaa gaacgttgat
      721 ttggctaatt acttctcacc atcgcctgcc attttccaat cgtctaaatt atggtgagaa
      781 cttcaaagaa aagacccaaa aggatattta tctgaatcaa gccaagtggg atgaatttta
      841 tcaatctcaa ttatatccga ccgagcaatg ggtaaaaaac gcccaaagta cgcatcctaa
      901 taaaaagcaa ttttggcaat ttgagcatct ggcgacagac agcctgtctt ggcaaaaagc
      961 aatgcgccgt tgggcaaaga aagcgctaaa tcatccgaca ttacttgcca tggatttttc
     1021 tgatcccttt ttcctgttag tgagtcgttt atgtttaatg gttggcgatc atcattattc
     1081 ttctctggat gctgatgtta atttggggga taacgatttt cgtgaaaaat taattgccaa
     1141 tacggataaa aaccatcgtg caaaacaagc actggacgaa catttgctcg gtgtttgtaa
     1201 aaccgctgtg tatttttccc gtttattgcc taaattgaag actgaactgc ctgcggttaa
     1261 acaacataaa ccctttgtca aacgaacgtc tattccgcgt tttcaatggc agaatccggc
     1321 atttgatttg gcaaaatccc ttcgggctga aacggaaaag tgcggtctgt tttcagtgaa
     1381 tttagccagt acggggtgtg gcaaaacttt gggtaatgcc cgcattatgt acgccttagc
     1441 ggatccgcaa aaaggggcgc gatttactgc tgcattaggc ttgcgtgttt taaccttgca
     1501 aacgggagaa gcgttacaga aaaagctaaa tttaacggag gatttactgg cgattttggt
     1561 cggcggaagt gcggtcaaaa aattatatga attgcagcaa tcgaaagaaa ataatggtag
     1621 cgaatctatt cagccatggt ttggggaaaa tgaagtgtat ggtgcggatt ttatcgactc
     1681 cgccattgaa gatgaaaaat ttgcggcgat tttacaggag cccaaagccc gaaaattatt
     1741 ttatgctccg atcgtgactt gtacggtaga tcatttaatt agtgcctctg aaacggcgcg
     1801 tggcggcaaa tatattgtgc cgatgttgcg tctgttaaca tcagatttaa tcttagatga
     1861 accggatgat tttaaccaag aagatttacc ggcattatcc cgtttagttc accttgccgg
     1921 tttatttggt tcccgcgtat tgctttcgtc ggcaacactg acaccggatt ttgttgcagg
     1981 actattcaac gcttatcaag ccggtcgggc gatttataat cgtcaacaag gaataaacca
     2041 agccgcgatt tgttgtgctt ggtttgatga gtttaagcaa acatcaacga attgtgcgaa
     2101 tcctgaggat ttcatgtttg cacatcaaag atttgtggag aaacgggctg aaaaattatg
     2161 taaagcatcg gtacgccgaa aagcacaaat tatgccgctt cctccattga aaaaaatcga
     2221 gaatcaatta tttgatgtcg taacctttgc cgaattttta gtacaacaaa ctattgattt
     2281 acataatgtt catagtgaat atgatcctgc gacacaaaaa caagtcagtt tcggtttaat
     2341 tcggtttgct aatattaagc cgatgattcc ccttgccaaa caacttttcc aaatggaaat
     2401 gaagccggca tggcaagatt atcaaattca tttatgttgt tatcattcca agcaattatt
     2461 gattttgcgc tcaagattag aagaaaagct tgatcgcatt ttgaatcgtg cggacagtaa
     2521 aaacgccttt acgcagcctg aagtgatggc tgcaatggca aactcacaaa ccaaacatca
     2581 gttatttatc gttttaggca gtccggtaac cgaggtggga cgggatcatg attacgattg
     2641 ggcgatcgtc gatccttcct caatgcgttc aattattcag cttgcaggac gtgtttggcg
     2701 acatcgaccg gataaagttg ctagccagcc gaatatcact ttattaccga ccaattggcg
     2761 cggtttagcc ggacaaaccg gtgtcatttt tactaagccg ggatttgaag atgcgaatca
     2821 tccgttagaa acacatcgta cagaagagtt aataccgaca gctcaattag agcgtattga
     2881 ttccattccc cgtattacaa aacgtttgga ttttcgaccg cacttttgct tagctgattt
     2941 agagcatcaa gcgacagcgg atttacttaa cagtcagaaa gtgaattgcg taaatgccta
     3001 ttggaataat gatttgagca atcgatttac agcaaactta cctttgatga cacgttttag
     3061 aaaatcacgc acacagcgca atattgtctg cttgttggat gaagacggaa agccttattt
     3121 cagagaacag gaaagtgcat gggaaacgcc ggataatcca caaaatgcac tgccggaatt
     3181 tcgttatcgg tcgttggacg gaaagggagg actggttaaa ccgtggttgg attcctcgct
     3241 acgtttggca ttaatggaac tggcggaacg gttagcggaa gaaagtttat tatttgttgc
     3301 gttgaaatat gcgacggtaa gcttggctga ggatgacacg cagtggtgtt ttaatgaaac
     3361 gctgggtttt tggcaggaag atc
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