LOCUS       BBa_K1773021            3381 bp    DNA     linear       04-May-2024
DEFINITION  Cas3 protein with pLuxcI right promoter and weak RBS.
ACCESSION   BBa_K1773021
VERSION     BBa_K1773021.1  
FEATURES             Location/Qualifiers
     promoter        1..78
                     /label=BBa_K1773015
     RBS             79..89
                     /label=RBS-4\Weaker
     RBS             85..88
                     /label=RBS
     misc_feature    79..89
                     /label=BBa_B0033
     RBS             79..89
                     /label=BBa_B0033
     promoter        1..89
                     /label=BBa_K1773018
     CDS             96..3381
                     /label=BBa_K1773003
ORIGIN
        1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga atacctctgg
       61 cggtgataca catgcatatc acacaggact actagatgaa tatcttactt gttagccaat
      121 gccaaaaaaa tgcgttaacg gaaacccgcc gcattctcga tcaatttgcc gaacggtgcg
      181 gtgatcgcgt atggcaaaca cccattacac aagctgggtt agacaccctg cataaaatgt
      241 tacgacaaac ggcgcgtaaa aatacagcag tcgcttgtta ttggacacat gggaaaaatt
      301 taacggaatt gctttggatt gtgggggata aatcacaatt taacgaagaa gggcgtgtgc
      361 cgacaaaccg aaccaagcgc aatattttgc gcgccgaaga tgaaggcggt tggttacaag
      421 cctattcgat ccaaattgtg accgcacttg ccgcgttatt acacgattta ggcaaggcga
      481 acaaagcctt tcagttaaaa ttgaaaccca atgcgccttt tcacgcggat tattttcgtc
      541 acgagtggat ttcagcccga ttgtttgaag ccatgattgc tggttgtaaa accgatcagg
      601 agtggctgtc acgtttggct aattgggctg aatataaaga aaattggtta gcttcgctta
      661 atattgatga taatgaagct gattttagtc atttgccgcc tttggcaaga acgttgattt
      721 ggctaattac ttctcaccat cgcctgccat tttccaatcg tctaaattat ggtgagaact
      781 tcaaagaaaa gacccaaaag gatatttatc tgaatcaagc caagtgggat gaattttatc
      841 aatctcaatt atatccgacc gagcaatggg taaaaaacgc ccaaagtacg catcctaata
      901 aaaagcaatt ttggcaattt gagcatctgg cgacagacag cctgtcttgg caaaaagcaa
      961 tgcgccgttg ggcaaagaaa gcgctaaatc atccgacatt acttgccatg gatttttctg
     1021 atcccttttt cctgttagtg agtcgtttat gtttaatggt tggcgatcat cattattctt
     1081 ctctggatgc tgatgttaat ttgggggata acgattttcg tgaaaaatta attgccaata
     1141 cggataaaaa ccatcgtgca aaacaagcac tggacgaaca tttgctcggt gtttgtaaaa
     1201 ccgctgtgta tttttcccgt ttattgccta aattgaagac tgaactgcct gcggttaaac
     1261 aacataaacc ctttgtcaaa cgaacgtcta ttccgcgttt tcaatggcag aatccggcat
     1321 ttgatttggc aaaatccctt cgggctgaaa cggaaaagtg cggtctgttt tcagtgaatt
     1381 tagccagtac ggggtgtggc aaaactttgg gtaatgcccg cattatgtac gccttagcgg
     1441 atccgcaaaa aggggcgcga tttactgctg cattaggctt gcgtgtttta accttgcaaa
     1501 cgggagaagc gttacagaaa aagctaaatt taacggagga tttactggcg attttggtcg
     1561 gcggaagtgc ggtcaaaaaa ttatatgaat tgcagcaatc gaaagaaaat aatggtagcg
     1621 aatctattca gccatggttt ggggaaaatg aagtgtatgg tgcggatttt atcgactccg
     1681 ccattgaaga tgaaaaattt gcggcgattt tacaggagcc caaagcccga aaattatttt
     1741 atgctccgat cgtgacttgt acggtagatc atttaattag tgcctctgaa acggcgcgtg
     1801 gcggcaaata tattgtgccg atgttgcgtc tgttaacatc agatttaatc ttagatgaac
     1861 cggatgattt taaccaagaa gatttaccgg cattatcccg tttagttcac cttgccggtt
     1921 tatttggttc ccgcgtattg ctttcgtcgg caacactgac accggatttt gttgcaggac
     1981 tattcaacgc ttatcaagcc ggtcgggcga tttataatcg tcaacaagga ataaaccaag
     2041 ccgcgatttg ttgtgcttgg tttgatgagt ttaagcaaac atcaacgaat tgtgcgaatc
     2101 ctgaggattt catgtttgca catcaaagat ttgtggagaa acgggctgaa aaattatgta
     2161 aagcatcggt acgccgaaaa gcacaaatta tgccgcttcc tccattgaaa aaaatcgaga
     2221 atcaattatt tgatgtcgta acctttgccg aatttttagt acaacaaact attgatttac
     2281 ataatgttca tagtgaatat gatcctgcga cacaaaaaca agtcagtttc ggtttaattc
     2341 ggtttgctaa tattaagccg atgattcccc ttgccaaaca acttttccaa atggaaatga
     2401 agccggcatg gcaagattat caaattcatt tatgttgtta tcattccaag caattattga
     2461 ttttgcgctc aagattagaa gaaaagcttg atcgcatttt gaatcgtgcg gacagtaaaa
     2521 acgcctttac gcagcctgaa gtgatggctg caatggcaaa ctcacaaacc aaacatcagt
     2581 tatttatcgt tttaggcagt ccggtaaccg aggtgggacg ggatcatgat tacgattggg
     2641 cgatcgtcga tccttcctca atgcgttcaa ttattcagct tgcaggacgt gtttggcgac
     2701 atcgaccgga taaagttgct agccagccga atatcacttt attaccgacc aattggcgcg
     2761 gtttagccgg acaaaccggt gtcattttta ctaagccggg atttgaagat gcgaatcatc
     2821 cgttagaaac acatcgtaca gaagagttaa taccgacagc tcaattagag cgtattgatt
     2881 ccattccccg tattacaaaa cgtttggatt ttcgaccgca cttttgctta gctgatttag
     2941 agcatcaagc gacagcggat ttacttaaca gtcagaaagt gaattgcgta aatgcctatt
     3001 ggaataatga tttgagcaat cgatttacag caaacttacc tttgatgaca cgttttagaa
     3061 aatcacgcac acagcgcaat attgtctgct tgttggatga agacggaaag ccttatttca
     3121 gagaacagga aagtgcatgg gaaacgccgg ataatccaca aaatgcactg ccggaatttc
     3181 gttatcggtc gttggacgga aagggaggac tggttaaacc gtggttggat tcctcgctac
     3241 gtttggcatt aatggaactg gcggaacggt tagcggaaga aagtttatta tttgttgcgt
     3301 tgaaatatgc gacggtaagc ttggctgagg atgacacgca gtggtgtttt aatgaaacgc
     3361 tgggtttttg gcaggaagat c
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