LOCUS       BBa_K1780017            1901 bp    DNA     linear       17-May-2024
DEFINITION  Killer Gene, induced by AHL
ACCESSION   BBa_K1780017
VERSION     BBa_K1780017.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..82
                     /label=prefix
     misc_feature    254..254
                     /label=T
     misc_feature    572..572
                     /label=A
     CDS             81..830
                     /label=luxR
     misc_feature    81..836
                     /label=BBa_C0062
     misc_feature    837..861
                     /label=Help:Barcodes
     CDS             81..836
                     /label=BBa_C0062
     stem_loop       881..924
                     /label=stem_loop
     misc_feature    870..949
                     /label=BBa_B0010
     terminator      870..949
                     /label=BBa_B0010
     stem_loop       965..984
                     /label=T7 TE
     misc_feature    991..991
                     /label=stop
     polyA_site      985..998
                     /label=polya
     misc_feature    958..998
                     /label=BBa_B0012
     terminator      958..998
                     /label=BBa_B0012
     terminator      870..998
                     /label=BBa_B0015
     misc_feature    1007..1026
                     /label=LuxR/HSL
     promoter        1026..1031
                     /label=-35
     promoter        1048..1053
                     /label=-10
     misc_feature    1059..1059
                     /label=start
     misc_feature    1007..1061
                     /label=BBa_R0062
     promoter        1007..1061
                     /label=BBa_R0062
     misc_feature    1074..1077
                     /label=conserved
     RBS             1070..1081
                     /label=BBa_B0034
     CDS             1118..1423
                     /label=ccdB (rev)
     CDS             1425..1645
                     /label=ccdA- (rev)
     misc_feature    1556..1556
                     /label=T to C mutation
     misc_feature    1684..1689
                     /label=-10 (rev)
     misc_feature    1707..1712
                     /label=-35 (rev)
     misc_feature    1090..1764
                     /label=BBa_P1010
     stem_loop       1784..1827
                     /label=stem_loop
     misc_feature    1773..1852
                     /label=BBa_B0010
     terminator      1773..1852
                     /label=BBa_B0010
     stem_loop       1868..1887
                     /label=T7 TE
     misc_feature    1894..1894
                     /label=stop
     polyA_site      1888..1901
                     /label=polya
     misc_feature    1861..1901
                     /label=BBa_B0012
     terminator      1861..1901
                     /label=BBa_B0012
     terminator      1773..1901
                     /label=BBa_B0015
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta
      121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta
      181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat
      241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta
      301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt
      361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga
      421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg
      481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg
      541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata
      601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg
      661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg
      721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta
      781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact
      841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc
      901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt
     1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagaga aagaggagaa
     1081 atactagaga ctggctgtgt ataagggagc ctgacattta tattccccag aacatcaggt
     1141 taatggcgtt tttgatgtca ttttcgcggt ggctgagatc agccacttct tccccgataa
     1201 cggagaccgg cacactggcc atatcggtgg tcatcatgcg ccagctttca tccccgatat
     1261 gcaccaccgg gtaaagttca cgggagactt tatctgacag cagacgtgca ctggccaggg
     1321 ggatcaccat ccgtcgcccg ggcgtgtcaa taatatcact ctgtacatcc acaaacagac
     1381 gataacggct ctctctttta taggtgtaaa ccttaaactg catttcacca gcccctgttc
     1441 tcgtcagcaa aagagccgtt catttcaata aaccgggcga cctcagccat cccttcctga
     1501 ttttccgctt tccagcgttc ggcacgcaga cgacgggctt cattctgcat ggttgtgctt
     1561 accagaccgg agatattgac atcatatatg ccttgagcaa ctgatagctg tcgctgtcaa
     1621 ctgtcactgt aatacgctgc ttcatagcat acctcttttt gacatacttc gggtatacat
     1681 atcagtatat attcttatac cgcaaaaatc agcgcgcaaa tacgcatact gttatctggc
     1741 ttttagtaag ccggatccac gcgttactag agccaggcat caaataaaac gaaaggctca
     1801 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
     1861 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
//