Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Joy R. L. Santos
Date created: 2015-09-17 11:00:00
Date modified: 2015-09-18 11:58:31
Modified Bacteria Lipoprotein
| Types | DnaRegion |
| Roles | sequence_feature Other |
| Sequences | BBa_K1787001_sequence (Version 1) |
Description
This part contains a T7 promoter with a Ribosome Binding Site next to it; additionally, it encodes for a Bacterial Modified Lipoprotein. We have preliminary data that suggests that after isolation, run through an SDS-PAGE, and silver staining, this protein shows a different colouration than that of the normal silver stained protein gels.Notes
In order for our sequence to be synthesized, we had to intercalate non-polar amino acids between the polar amino acids. This, in order to make the protein that is synthesized by the sequence more reactive with the silver staining reagents.Source
The DNA sequence encoding for the protein was modified so as to reduce non-polar amino acids in between the polar amino-acids. It was introduced into BBa_K1321338 (containing a T7 promoter with a RBS).| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1321338 Scar (SpeI/XbaI) Modified Bacteria Lipoprotein | 1,43 44,51 52,763 | feature/BioBrick engineered_region feature/scar CDS feature/protein |