LOCUS       BBa_K1791002             826 bp    DNA     linear       17-May-2024
DEFINITION  Low RBS expressing MS2 Coat protein ribozyme affinity purification 
            (RAP)
ACCESSION   BBa_K1791002
VERSION     BBa_K1791002.1  
FEATURES             Location/Qualifiers
     promoter        1..23
                     /label=BBa_I719005
     misc_feature    38..41
                     /label=conserved
     RBS             32..45
                     /label=BBa_B0031
     CDS             52..459
                     /label=BBa_K1791003
     stem_loop       479..522
                     /label=stem_loop
     misc_feature    468..547
                     /label=BBa_B0010
     terminator      468..547
                     /label=BBa_B0010
     stem_loop       563..582
                     /label=T7 TE
     misc_feature    589..589
                     /label=stop
     polyA_site      583..596
                     /label=polya
     misc_feature    556..596
                     /label=BBa_B0012
     terminator      556..596
                     /label=BBa_B0012
     terminator      468..596
                     /label=BBa_B0015
     misc_feature    605..610
                     /label=BBa_K1791007
     misc_feature    619..767
                     /label=BBa_K1791005
     misc_feature    776..781
                     /label=BBa_K1791008
     misc_feature    790..826
                     /label=BBa_K1791006
ORIGIN
        1 taatacgact cactataggg agatactaga gtcacacagg aaacctacta gatggcaagc
       61 aacttcacgc agtttgtgct ggtcgacaat ggcggtaccg gtgacgttac ggttgcccca
      121 agcaacttcg ccaacggtgt cgccgagtgg atttccagca actctcgctc ccaggcatat
      181 aaagtaacat gtagcgtgcg ccagtcaagt gcacagaatc gcaagtacac catcaaagtg
      241 gaggtgccga aagtcgcaac ccagacggtg ggcggtgttg aacttccggt tgccgcgtgg
      301 cgctcctatc tgaatatgga actgaccatc ccgatctttg ctaccaattc cgattgcgaa
      361 ttgattgtca aagcgatgca ggggttactg aaggacggaa atccgattcc aagtgcaatt
      421 gctgctaaca gcggaatcta tcaccaccac catcatcatt actagagcca ggcatcaaat
      481 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
      541 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact
      601 agagggatcc tactagagta atacgactca ctatagggag aaaacaaaca aagctgtcac
      661 cggatgtgct ttccggtctg atgagtccgt gttgctgata ccagcatcgt cttgatgccc
      721 ttggcagcag tggacgagga cgaaacagca aaaagaaaaa taaaaaatac tagagggtac
      781 ctactagagg aaaagaaaac atgaggatca cccatgtcaa aagaaa
//