BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_K1791002
1
BBa_K1791002
Low RBS expressing MS2 Coat protein ribozyme affinity purification (RAP)
2015-09-16T11:00:00Z
2015-09-23T01:34:01Z
This is an amalgamation of several biobricks an MS2 coat protein, an MS2 binding domain and the theophylline ribozyme (BBa_K1791000)
low RBS expressing MS2 Coat protein ribozyme affinity purification (RAP). This part utilizes the expression of C-terminal 6X His tagged fusion MS2 coat protein under a T7 promoter and a low efficiency shine dalgarno sequence for expression of MS2. Downstream there is a T7 promoter followed by a theophylline ribozyme and a MS2 apatamer at the 3' end. Co-expression of the two modules results in MS2 binding to the 3' end of the theophylline ribozyme transcript allowing for the MS2-RNA purification by nickel affinity chromatography. The RNA can be eluted from the complex by the addition of theophylline.
false
false
_2216_
17031
17031
9
false
We had to consider MS2 expression levels relative to the theophylline ribozyme transcript and assessed how this affects total RNA yield. In order to test this we developed a second part with increased MS2 expression (BBa_K1791001)
false
Graeme Glaister
component2476629
1
BBa_K1791006
component2476628
1
BBa_K1791008
component2476626
1
BBa_K1791007
component2476617
1
BBa_B0031
component2476625
1
BBa_B0015
component2476615
1
BBa_I719005
component2476618
1
BBa_K1791003
component2476627
1
BBa_K1791005
annotation2476625
1
BBa_B0015
range2476625
1
468
596
annotation2476629
1
BBa_K1791006
range2476629
1
790
826
annotation2476626
1
BBa_K1791007
range2476626
1
605
610
annotation2476615
1
BBa_I719005
range2476615
1
1
23
annotation2476628
1
BBa_K1791008
range2476628
1
776
781
annotation2476617
1
BBa_B0031
range2476617
1
32
45
annotation2476618
1
BBa_K1791003
range2476618
1
52
459
annotation2476627
1
BBa_K1791005
range2476627
1
619
767
BBa_B0031
1
BBa_B0031
RBS.2 (weak) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Medium RBS based on Ron Weiss thesis. Strength considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
<P> <P>Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-1" in figure 4-14 of thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Cho</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23316
1
conserved
range23316
1
7
10
BBa_I719005
1
pT7
T7 Promoter
2007-10-23T11:00:00Z
2015-08-31T04:07:53Z
---
Released HQ 2013
Just a T7 Promoter
false
false
_128_
0
2097
9
In stock
true
None
true
Imperial 2007
BBa_K1791007
1
BBa_K1791007
BamHI cut site
2015-09-22T11:00:00Z
2015-09-23T01:21:53Z
https://www.neb.com/products/r0136-bamhi
This site will be utilized in the composite part BBa_K1791001 and BBa_K1791002 in order to allow for insertion of new RNA strands to be generated in these constructs.
false
false
_2216_
17031
17031
9
false
no addtional design features were considered
false
Graeme Glaister
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916612
1
BBa_B0012
component1916610
1
BBa_B0010
annotation1916612
1
BBa_B0012
range1916612
1
89
129
annotation1916610
1
BBa_B0010
range1916610
1
1
80
BBa_K1791006
1
BBa_K1791006
MS2 binding domain
2015-09-22T11:00:00Z
2015-09-22T11:21:29Z
genomic sequence in E.coli
This is an aptamer designed to bind MS2 coat protein upon transcription.
false
false
_2216_
17031
17031
9
false
Had to test that it optimally binds to MS2 coat protein in vivo
false
Graeme Glaister
BBa_K1791005
1
BBa_K1791005
T7 promoter fused with a theophylline inducible ribozyme (aptazyme)
2015-09-22T11:00:00Z
2015-09-22T11:17:58Z
Win, M.N., Smolke C.D. 2007 and BBa_I719005
This part was developed in order to increase the sequence space flexibility in dsRNA generated using BBa_K1791001 and BBa_K1791002
false
false
_2216_
17031
17031
9
false
by changing the T7 promoter sequence (BBa_I719005) slightly after the GGG trinulceotide we get an increase in the potential for generating RNA with our composite parts BBa_K1791001 and BBa_K1791002.
false
Graeme Glaister
BBa_K1791008
1
BBa_K1791008
KpnI cut site
2015-09-22T11:00:00Z
2015-09-23T01:29:59Z
https://www.neb.com/products/r0142-kpni
This site will be utilized in the composite part BBa_K1791001 and BBa_K1791002 in order to allow for insertion of new RNA strands to be generated in these constructs.
false
false
_2216_
17031
17031
9
false
no additional design considerations were neccessary
false
Graeme Glaister
BBa_K1791003
1
BBa_K1791003
6X end terminally His tagged MS2 coat protein
2015-09-22T11:00:00Z
2015-09-22T11:10:08Z
RefSeq NP_040648.1. NC_001417.2.
MS2 coat protein can be easily purified via a nickel sepharose purification if this part is used downstream of a promoter and RBS.
false
false
_2216_
17031
17031
9
false
optimized for expression in E.coli
false
Graeme Glaister
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_I719005_sequence
1
taatacgactcactatagggaga
BBa_K1791008_sequence
1
ggtacc
BBa_K1791003_sequence
1
atggcaagcaacttcacgcagtttgtgctggtcgacaatggcggtaccggtgacgttacggttgccccaagcaacttcgccaacggtgtcgccgagtggatttccagcaactctcgctcccaggcatataaagtaacatgtagcgtgcgccagtcaagtgcacagaatcgcaagtacaccatcaaagtggaggtgccgaaagtcgcaacccagacggtgggcggtgttgaacttccggttgccgcgtggcgctcctatctgaatatggaactgaccatcccgatctttgctaccaattccgattgcgaattgattgtcaaagcgatgcaggggttactgaaggacggaaatccgattccaagtgcaattgctgctaacagcggaatctatcaccaccaccatcatcat
BBa_K1791006_sequence
1
gaaaagaaaacatgaggatcacccatgtcaaaagaaa
BBa_K1791002_sequence
1
taatacgactcactatagggagatactagagtcacacaggaaacctactagatggcaagcaacttcacgcagtttgtgctggtcgacaatggcggtaccggtgacgttacggttgccccaagcaacttcgccaacggtgtcgccgagtggatttccagcaactctcgctcccaggcatataaagtaacatgtagcgtgcgccagtcaagtgcacagaatcgcaagtacaccatcaaagtggaggtgccgaaagtcgcaacccagacggtgggcggtgttgaacttccggttgccgcgtggcgctcctatctgaatatggaactgaccatcccgatctttgctaccaattccgattgcgaattgattgtcaaagcgatgcaggggttactgaaggacggaaatccgattccaagtgcaattgctgctaacagcggaatctatcaccaccaccatcatcattactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagggatcctactagagtaatacgactcactatagggagaaaacaaacaaagctgtcaccggatgtgctttccggtctgatgagtccgtgttgctgataccagcatcgtcttgatgcccttggcagcagtggacgaggacgaaacagcaaaaagaaaaataaaaaatactagagggtacctactagaggaaaagaaaacatgaggatcacccatgtcaaaagaaa
BBa_B0031_sequence
1
tcacacaggaaacc
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K1791007_sequence
1
ggatcc
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K1791005_sequence
1
taatacgactcactatagggagaaaacaaacaaagctgtcaccggatgtgctttccggtctgatgagtccgtgttgctgataccagcatcgtcttgatgcccttggcagcagtggacgaggacgaaacagcaaaaagaaaaataaaaaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z