LOCUS       BBa_K1795022            2258 bp    DNA     linear       14-Jun-2024
DEFINITION  R0010 CFP/YFP Dual Reporter Plasmid
ACCESSION   BBa_K1795022
VERSION     BBa_K1795022.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..988
                     /label=CFP (LVA)
     misc_feature    945..982
                     /label=SsrA
     misc_feature    983..988
                     /label=2
     misc_feature    295..295
                     /label=A
     misc_feature    227..988
                     /label=BBa_E0022
     misc_feature    227..988
                     /label=BBa_E0022
     stem_loop       1008..1051
                     /label=stem_loop
     misc_feature    997..1076
                     /label=BBa_B0010
     terminator      997..1076
                     /label=BBa_B0010
     stem_loop       1092..1111
                     /label=T7 TE
     misc_feature    1118..1118
                     /label=stop
     polyA_site      1112..1125
                     /label=polya
     misc_feature    1085..1125
                     /label=BBa_B0012
     terminator      1085..1125
                     /label=BBa_B0012
     terminator      997..1125
                     /label=BBa_B0015
     CDS             1134..1221
                     /label=end of LacI coding region (inactive)
     misc_feature    1134..1333
                     /label=BBa_R0010
     misc_feature    1222..1259
                     /label=CAP binding site
     promoter        1270..1275
                     /label=-35
     promoter        1294..1299
                     /label=-10
     misc_feature    1299..1333
                     /label=LacI binding site
     misc_feature    1306..1306
                     /label=start
     promoter        1134..1333
                     /label=BBa_R0010
     misc_feature    1346..1349
                     /label=conserved
     RBS             1342..1353
                     /label=BBa_B0034
     CDS             1360..2121
                     /label=YFP (LVA)
     misc_feature    2116..2121
                     /label=2
     misc_feature    2077..2115
                     /label=SsrA
     misc_feature    1601..1601
                     /label=A (Q->L)
     misc_feature    1360..2121
                     /label=BBa_E0032
     misc_feature    1360..2121
                     /label=BBa_E0032
     stem_loop       2141..2184
                     /label=stem_loop
     misc_feature    2130..2209
                     /label=BBa_B0010
     terminator      2130..2209
                     /label=BBa_B0010
     stem_loop       2225..2244
                     /label=T7 TE
     misc_feature    2251..2251
                     /label=stop
     polyA_site      2245..2258
                     /label=polya
     misc_feature    2218..2258
                     /label=BBa_B0012
     terminator      2218..2258
                     /label=BBa_B0012
     terminator      2130..2258
                     /label=BBa_B0015
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg tgagcaaggg
      241 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtgaacgg
      301 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
      361 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
      421 gacctggggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
      481 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
      541 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
      601 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
      661 ctacatcagc cacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa
      721 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
      781 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
      841 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
      901 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagaggcctg ctgcaaacga
      961 cgaaaactac gctttagtag cttaataata ctagagccag gcatcaaata aaacgaaagg
     1021 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
     1081 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagcaatacg
     1141 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     1201 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     1261 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     1321 acaatttcac acatactaga gaaagaggag aaatactaga tggtgagcaa gggcgaggag
     1381 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     1441 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     1501 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cttcggctac
     1561 ggcctgcaat gcttcgcccg ctaccccgac cacatgaagc tgcacgactt cttcaagtcc
     1621 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     1681 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     1741 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     1801 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     1861 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     1921 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcta ccagtccgcc
     1981 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     2041 gccgggatca ctctcggcat ggacgagctg tacaagaggc ctgctgcaaa cgacgaaaac
     2101 tacgctttag tagcttaata atactagagc caggcatcaa ataaaacgaa aggctcagtc
     2161 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
     2221 cactggctca ccttcgggtg ggcctttctg cgtttata
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