Pnif

BBa_K1796001 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1796001
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nannan Xie
Date created: 2015-09-16 11:00:00
Date modified: 2015-09-18 02:35:42

promoter of Paenibacillus sp. WLY78 nif gene cluster



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_K1796001_sequence (Version 1)

Description

The promoter of Paenibacillus sp. WLY78 nif gene cluster, We named it Pnif. Pnif is a σ70-dependent promoter located upstream of nifB. Pnif is in control of all the gene clusters of nitrogen fixation genome.
Confirmation of nif promoter
We standardized the nif promoter of Paenibacillus sp. WLY78, and linked it to the upstream of 3 reporter genes (RFP reporter: BBa_K1357010, YFP reporter: BBa_E0430;amilCP reporter: BBa_K1357009) to confirm the reliability of this promoter. The results are shown in Figure 2.
A B C

http://2015.igem.org/wiki/images/b/b2/Confirmation_of_Pnif_AB.png
http://2015.igem.org/wiki/images/thumb/3/34/C-confirmation_of_Pnif.jpeg/622px-C-confirmation_of_Pnif.jpeg
Fig.4. Confirmation of nif promoter of Paenibacillus sp. WLY78. (A) Pnif+RFP reporter; (B) Pnif+YFP reporter;(C)Pnif+amilCP reporter.

The results confirmed the reliability of Pnif. The initiation of Pnif does not require inducement. Thus it is a effective constitutive promoter.

Notes

effeciency of the promoter in E.coli for ordinary parts

Source

Pnif is from Paenibacillus sp. WLY78 genome,located upstream of nifB,the first gene of nitrogen fixation cluster.

Sequence Annotation Location Component / Role(s)
-35
-10
Pnif
62,67
85,90
1,100
operator feature/operator
feature/operator operator
feature/promoter promoter
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BBa_K1796001/1