Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Laura Lee Irvin
Date created: 2015-08-24 11:00:00
Date modified: 2015-08-31 11:29:06
Mambalgin-1 for E. coli
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1800000_sequence (Version 1) |
Description
This codes for the peptide Mambalgin-1, an analgesic. Mambalgin is a protein component of the venom of Dendroaspis polylepis, better known as the Black Mamba. Mambalgin-1 peptide that is a powerful analgesic that directly blocks pain transmission in the peripheral nervous system (Diochot et al, 2012) by targeting acid-sensing ion channels within nociceptors beneath the epidermis. Because Mambalgin acts on pain receptors within the skin rather than on opioid receptors in the brain, this peptide has great potential as a medication for pain treatment that is non-addicting and non-habit forming. Furthermore, recombinant purification of Mambalgin could assist in developing anti-venom without the attendant risk of harvesting venom directly from snakes. This part is designed for expression in E. coli.Notes
For the design, we used assembly standard 10 and also included a start codon in the Mambalgin-1 cDNA sequence and had it synthesized with an RBS region, lacI promoter region, MYC epitome, and 6xHis tag for optimized expression in E. coli.Source
Mambalgin-1 sequence (BBa_k1110003), RBS (BBa_j61101), lacI Promoter (BBa_R0011), MYC and 6xHis tag was copied from the pGAPZα vector sequence.| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0011 lac O1 -35 lac O1 -10 BBa_J61101 Mambalgin codon optimized for E. coli MYC Epitope 6x His Tag | 1,54 3,19 20,25 26,42 43,48 64,75 84,254 258,287 303,320 | engineered_region feature/BioBrick feature/operator operator feature/promoter promoter operator feature/operator promoter feature/promoter engineered_region feature/BioBrick CDS feature/cds tag feature/tag feature/tag tag |