Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Richard Wilson, Stephanie Chambers
Date created: 2009-10-15 11:00:00
Date modified: 2015-05-08 01:11:06
Game of Life - Primary plasmid
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K180004_sequence (Version 1) |
Description
Composite part, consisting of BBa_K180005 and BBa_K180006, but with BBa_K180005 reading along reverse strand, and BBa_K180006 reading along the forward strand.Notes
BBa_K180005 reading along reverse strand, and BBa_K180006 reading along the forward strand to ensure the constitutive promoter BBa_J23119 (present in BBa_K180005) has no effect over LacI or GFP-LVA expression in BBa_K180006.Source
See relevant biobrick component parts (BBa_K180005 and BBa_K180006)| Sequence Annotation | Location | Component / Role(s) |
| K180005 B0015 C0062 luxR B0034 conserved J23119 -10 LuxR/HSL -35 R0062 K180006 -10 start C0012 lacI B0034 conserved LVA GFPmut3b K180001 B0034 conserved LVA B0015 -35 | 1,979 1,129 163,918 163,912 925,936 929,932 945,979 951,956 988,1007 974,979 988,1042 988,3138 1029,1034 1040,1040 1069,2196 1069,2157 1051,1062 1055,1058 2158,2196 2248,2967 2248,3001 2230,2241 2234,2237 2968,3001 3010,3138 1007,1012 | feature/BioBrick engineered_region feature/BioBrick engineered_region feature/BioBrick engineered_region CDS feature/cds engineered_region feature/BioBrick feature/conserved conserved_region feature/BioBrick engineered_region feature/promoter promoter feature/binding non_covalent_binding_site promoter feature/promoter feature/BioBrick engineered_region feature/BioBrick engineered_region promoter feature/promoter start_codon feature/start engineered_region feature/BioBrick CDS feature/cds engineered_region feature/BioBrick conserved_region feature/conserved tag feature/tag CDS feature/protein engineered_region feature/BioBrick feature/BioBrick engineered_region feature/conserved conserved_region tag feature/tag engineered_region feature/BioBrick feature/promoter promoter |