LOCUS       BBa_K180004             3138 bp    DNA     linear       19-Apr-2024
DEFINITION  Game of Life - Primary plasmid
ACCESSION   BBa_K180004
VERSION     BBa_K180004.1  
FEATURES             Location/Qualifiers
     misc_feature    1..979
                     /label=K180005
     misc_feature    1..129
                     /label=B0015
     misc_feature    163..918
                     /label=C0062
     CDS             163..912
                     /label=luxR
     misc_feature    925..936
                     /label=B0034
     misc_feature    929..932
                     /label=conserved
     misc_feature    945..979
                     /label=J23119
     promoter        951..956
                     /label=-10
     misc_feature    988..1007
                     /label=LuxR/HSL
     promoter        974..979
                     /label=-35
     misc_feature    988..1042
                     /label=R0062
     misc_feature    988..3138
                     /label=K180006
     promoter        1029..1034
                     /label=-10
     misc_feature    1040..1040
                     /label=start
     misc_feature    1069..2196
                     /label=C0012
     CDS             1069..2157
                     /label=lacI
     misc_feature    1051..1062
                     /label=B0034
     misc_feature    1055..1058
                     /label=conserved
     misc_feature    2158..2196
                     /label=LVA
     CDS             2248..2967
                     /label=GFPmut3b
     misc_feature    2248..3001
                     /label=K180001
     misc_feature    2230..2241
                     /label=B0034
     misc_feature    2234..2237
                     /label=conserved
     misc_feature    2968..3001
                     /label=LVA
     misc_feature    3010..3138
                     /label=B0015
     promoter        1007..1012
                     /label=-35
ORIGIN
        1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca
       61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
      121 gatgcctggc tctagtagtg atctacacta gcactatcag tgttattaat ttttaaagta
      181 tgggcaatca attgctcctg ttaaaattgc tttagaaata ctttggcagc ggtttgttgt
      241 attgagtttc atttgcgcat tggttaaatg gaaagtgaca gtacgctcac tgcaacctaa
      301 tatttttgaa atatcccaag agctttttcc ttcgcatgcc cacgctaaac attctttttc
      361 tcttttggtt aaatcgttgt ttgatttatt atttgctata tttatttttc gataattatc
      421 aactagagaa ggaacaatta atggtatgtt catacacgca tgtaaaaata aactatctat
      481 atagttgtct ttttctgaat gtgcaaaact aagcattccg aagccattgt tagccgtatg
      541 aatagggaaa ctaaacccag tgataagacc tgatgttttc gcttctttaa ttacatttgg
      601 agatttttta tttacagcat tgttttcaaa tatattccaa ttaattggtg aatgattgga
      661 gttagaataa tctactatag gatcatattt tattaaatta gcgtcatcat aatattgcct
      721 ccatttttta gggtaattat ctaggattga aatatcagat ttaaccatag aatgaggata
      781 aatgatcgcg agtaaataat attcacaatg taccatttta gtcatatcag ataagcattg
      841 attaatatca ttattgcttc tacaagcttt aattttatta attattctgt atgtgtcgtc
      901 ggcatttatg tttttcatct agtatttctc ctctttctct agtagctagc attataccta
      961 ggactgagct agctgtcaat actagagacc tgtaggatcg tacaggttta cgcaagaaaa
     1021 tggtttgtta tagtcgaata aatactagag aaagaggaga aatactagat ggtgaatgtg
     1081 aaaccagtaa cgttatacga tgtcgcagag tatgccggtg tctcttatca gaccgtttcc
     1141 cgcgtggtga accaggccag ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg
     1201 atggcggagc tgaattacat tcccaaccgc gtggcacaac aactggcggg caaacagtcg
     1261 ttgctgattg gcgttgccac ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg
     1321 gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga
     1381 agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg
     1441 ctgatcatta actatccgct ggatgaccag gatgccattg ctgtggaagc tgcctgcact
     1501 aatgttccgg cgttatttct tgatgtctct gaccagacac ccatcaacag tattattttc
     1561 tcccatgaag acggtacgcg actgggcgtg gagcatctgg tcgcattggg tcaccagcaa
     1621 atcgcgctgt tagcgggccc attaagttct gtctcggcgc gtctgcgtct ggctggctgg
     1681 cataaatatc tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg cgactggagt
     1741 gccatgtccg gttttcaaca aaccatgcaa atgctgaatg agggcatcgt tcccactgcg
     1801 atgctggttg ccaacgatca gatggcgctg ggcgcaatgc gcgccattac cgagtccggg
     1861 ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg ataccgaaga cagctcatgt
     1921 tatatcccgc cgttaaccac catcaaacag gattttcgcc tgctggggca aaccagcgtg
     1981 gaccgcttgc tgcaactctc tcagggccag gcggtgaagg gcaatcagct gttgcccgtc
     2041 tcactggtga aaagaaaaac caccctggcg cccaatacgc aaaccgcctc tccccgcgcg
     2101 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcaggct
     2161 gcaaacgacg aaaactacgc tttagtagct taataactct gatagtgcta gtgtagatct
     2221 ctactagaga aagaggagaa atactagatg cgtaaaggag aagaactttt cactggagtt
     2281 gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga
     2341 gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga
     2401 aaactacctg ttccatggcc aacacttgtc actactttcg gttatggtgt tcaatgcttt
     2461 gcgagatacc cagatcatat gaaacagcat gactttttca agagtgccat gcccgaaggt
     2521 tatgtacagg aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa
     2581 gtcaagtttg aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa
     2641 gaagatggaa acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac
     2701 atcatggcag acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt
     2761 gaagatggaa gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc
     2821 cctgtccttt taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc
     2881 aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat
     2941 ggcatggatg aactatacaa ataataagct gcaaacgacg aaaactacgc tttagtagct
     3001 ttactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     3061 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
     3121 ggcctttctg cgtttata
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