LOCUS       BBa_K180006             2151 bp    DNA     linear       24-Apr-2024
DEFINITION  Game of Life - Primary plasmid (part 2) [lux pR, GFP and LacI 
            generator]
ACCESSION   BBa_K180006
VERSION     BBa_K180006.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        20..25
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    53..53
                     /label=start
     misc_feature    1..55
                     /label=BBa_R0062
     promoter        1..55
                     /label=BBa_R0062
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    1171..1209
                     /label=LVA
     CDS             82..1209
                     /label=lacI-LVA
     misc_feature    82..1209
                     /label=BBa_C0012
     misc_feature    1210..1234
                     /label=Help:Barcodes
     CDS             82..1209
                     /label=BBa_C0012
     misc_feature    1247..1250
                     /label=conserved
     RBS             1243..1254
                     /label=BBa_B0034
     CDS             1261..1980
                     /label=GFPmut3b
     misc_feature    1981..2014
                     /label=LVA
     misc_feature    1261..2014
                     /label=K180001
     CDS             1261..2014
                     /label=BBa_K180001
     stem_loop       2034..2077
                     /label=stem_loop
     misc_feature    2023..2102
                     /label=BBa_B0010
     terminator      2023..2102
                     /label=BBa_B0010
     stem_loop       2118..2137
                     /label=T7 TE
     misc_feature    2144..2144
                     /label=stop
     polyA_site      2138..2151
                     /label=polya
     misc_feature    2111..2151
                     /label=BBa_B0012
     terminator      2111..2151
                     /label=BBa_B0012
     terminator      2023..2151
                     /label=BBa_B0015
ORIGIN
        1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta
       61 gagaaagagg agaaatacta gatggtgaat gtgaaaccag taacgttata cgatgtcgca
      121 gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt
      181 tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac
      241 cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt
      301 ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg
      361 ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg
      421 gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac
      481 caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc
      541 tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc
      601 gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt
      661 tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt
      721 cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg
      781 caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg
      841 ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta
      901 gtgggatacg acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa
      961 caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc
     1021 caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg
     1081 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     1141 cgacaggttt cccgactgga aagcgggcag gctgcaaacg acgaaaacta cgctttagta
     1201 gcttaataac tctgatagtg ctagtgtaga tctctactag agaaagagga gaaatactag
     1261 atgcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
     1321 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga
     1381 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt
     1441 gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag
     1501 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc
     1561 aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt
     1621 aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa
     1681 ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga
     1741 atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac
     1801 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
     1861 ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt
     1921 cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caaataataa
     1981 gctgcaaacg acgaaaacta cgctttagta gctttactag agccaggcat caaataaaac
     2041 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     2101 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
//