BBa_K180009

BBa_K180009 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K180009
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Richard Wilson, Stephanie Chambers
Date created: 2009-10-15 11:00:00
Date modified: 2016-01-25 01:37:03

mBanana (rights owned by Clontech)



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K180009_sequence (Version 1)

Description

mBanana fluorescent protein - Rights owned by Clontech. Sequence is commercially available from Clontech. Sequence obtained through PCR amplification, with BioBrick prefix and suffix added through in-house designed primers.

Notes

PstI site within the mBanana sequence - has to be assembled using BioBrick assembly in such an order that any successive digestions will not require PstI, otherwise the mCherry sequence will be cut as well as the site of interest. Alternatively, the assembly can be performed using non-standard BioBrick assembly.
Part cannot be submitted to registry as the rights are owned by Clontech. The mCherry sequence is however commercially available from Clontech in the pmBanana vector.

Source

pmBanana vector, Clontech

Sequence Annotation Location Component / Role(s)
mBanana
1,708
feature/protein CDS
igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K180009/1