Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Richard Wilson, Stephanie Chambers
Date created: 2009-10-20 11:00:00
Date modified: 2015-05-08 01:11:06
Rock-paper-scissors - Paper primary plasmid
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K180014_sequence (Version 1) |
Description
Composite part, consisting of BBa_K180005 and BBa_K180015, but with BBa_K180005 reading along the reverse strand, and BBa_K180015 reading along the forward strand.Forms the 'paper' primary plasmid in Team:Southampton's rock-paper-scissors project design.
Notes
BBa_K180005 reading along reverse strand, and BBa_K180015 reading along the forward strand to ensure the constitutive promoter BBa_J23119 (present in BBa_K180005) has no effect over GFP or RhlI expression in BBa_K180015.Source
See relevant biobrick component parts (BBa_K180011 and BBa_K180012).| Sequence Annotation | Location | Component / Role(s) |
| K180005 K180015 B0015 C0062 luxR B0034 conserved B0034 conserved B0034 conserved J23119 -35 -10 K180000 lacIq Ptac E0040 GFPmut3b C0170 rhlI B0015 | 1,979 988,3952 1,129 163,918 169,918 2443,2454 2447,2450 3189,3200 3193,3196 925,936 929,932 945,979 975,979 951,956 988,2434 988,2069 2312,2339 2461,3180 2461,3180 3207,3815 3207,3815 3824,3952 | feature/BioBrick engineered_region engineered_region feature/BioBrick engineered_region feature/BioBrick engineered_region feature/BioBrick CDS feature/cds engineered_region feature/BioBrick feature/conserved conserved_region engineered_region feature/BioBrick feature/conserved conserved_region engineered_region feature/BioBrick feature/conserved conserved_region feature/BioBrick engineered_region promoter feature/promoter feature/promoter promoter engineered_region feature/BioBrick CDS feature/protein promoter feature/promoter engineered_region feature/BioBrick CDS feature/protein engineered_region feature/BioBrick CDS feature/cds engineered_region feature/BioBrick |