LOCUS       BBa_K180015             2965 bp    DNA     linear       09-May-2024
DEFINITION  Paper - Primary plasmid (part 2) [tac pR, GFP and RhlI generator]
ACCESSION   BBa_K180015
VERSION     BBa_K180015.1  
FEATURES             Location/Qualifiers
     CDS             1..1082
                     /label=lacIq
     promoter        1325..1352
                     /label=Ptac
     promoter        1..1447
                     /label=BBa_K180000
     misc_feature    1460..1463
                     /label=conserved
     RBS             1456..1467
                     /label=BBa_B0034
     CDS             1474..2193
                     /label=GFP protein
     CDS             1474..2193
                     /label=BBa_E0040
     misc_feature    2206..2209
                     /label=conserved
     RBS             2202..2213
                     /label=BBa_B0034
     CDS             2220..2828
                     /label=rhII
     CDS             2220..2828
                     /label=BBa_C0170
     stem_loop       2848..2891
                     /label=stem_loop
     misc_feature    2837..2916
                     /label=BBa_B0010
     terminator      2837..2916
                     /label=BBa_B0010
     stem_loop       2932..2951
                     /label=T7 TE
     misc_feature    2958..2958
                     /label=stop
     polyA_site      2952..2965
                     /label=polya
     misc_feature    2925..2965
                     /label=BBa_B0012
     terminator      2925..2965
                     /label=BBa_B0012
ORIGIN
        1 gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt
       61 tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg
      121 gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag
      181 tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc
      241 gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa
      301 cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt
      361 gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc
      421 actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt
      481 ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag
      541 caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc
      601 tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg
      661 agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact
      721 gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc
      781 gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca
      841 tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc
      901 gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc
      961 gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc
     1021 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     1081 tgagcgcaac gcaattaatg taagttagct cactcattag gcacaattct catgtttgac
     1141 agcttatcat cgactgcacg gtgcaccaat gcttctggcg tcaggcagcc atcggaagct
     1201 gtggtatggc tgtgcaggtc gtaaatcact gcataattcg tgtcgctcaa ggcgcactcc
     1261 cgttctggat aatgtttttt gcgccgacat cataacggtt ctggcaaata ttctgaaatg
     1321 agctgttgac aattaatcat cggctcgtat aatgtgtgga attgtgagcg gataacaatt
     1381 tcacacagga aacagccagt ccgtttaggt gttttcacga gcacttcacc aacaaggacc
     1441 atagcattac tagagaaaga ggagaaatac tagatgcgta aaggagaaga acttttcact
     1501 ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc
     1561 agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact
     1621 actggaaaac tacctgttcc atggccaaca cttgtcacta ctttcggtta tggtgttcaa
     1681 tgctttgcga gatacccaga tcatatgaaa cagcatgact ttttcaagag tgccatgccc
     1741 gaaggttatg tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt
     1801 gctgaagtca agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat
     1861 tttaaagaag atggaaacat tcttggacac aaattggaat acaactataa ctcacacaat
     1921 gtatacatca tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa aattagacac
     1981 aacattgaag atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc
     2041 gatggccctg tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa
     2101 gatcccaacg aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt
     2161 acacatggca tggatgaact atacaaataa taatactaga gaaagaggag aaatactaga
     2221 tgatcgaact gctgtccgaa tccctggaag gtctgtccgc tgctatgatc gctgaactgg
     2281 gtcgttaccg tcaccaggtt ttcatcgaaa aactgggttg ggacgttgtt tccacctccc
     2341 gtgttcgtga ccaggagttc gaccagttcg accacccgca gacccgttac atcgttgcta
     2401 tgtcccgtca gggtatctgc ggttgcgctc gtctgctgcc gaccaccgac gcttacctgc
     2461 tgaaagacgt tttcgcttac ctgtgctccg aaaccccgcc gtccgacccg tccgtttggg
     2521 aactgtcccg ttacgctgct tccgctgctg acgacccgca gctggctatg aaaatcttct
     2581 ggtcctccct ccagtgcgct tggtacctgg gtgcttcctc cgttgttgct gttaccacca
     2641 ccgctatgga acgttacttc gttcgtaacg gtgttatcct ccagcgtctg ggtccgccgc
     2701 agaaagttaa aggtgaaacc ctggttgcta tctccttccc ggcttaccag gaacgtggtc
     2761 tggaaatgct gctgcgttac cacccggaat ggctccaggg tgttccgctg tccatggctg
     2821 tttaataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     2881 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
     2941 tcgggtgggc ctttctgcgt ttata
//